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The PFOS disturbed immunomodulatory functions via nuclear Factor-κB signaling in liver of zebrafish (Danio rerio)
- Source :
- Fishshellfish immunology. 91
- Publication Year :
- 2018
-
Abstract
- Excessive perfluorooctane sulfonate (PFOS) in natural water ecosystem has the potential to detrimentally affect immune system, but little is known of such effects or underlying mechanisms in fish. In the present study, we evaluated the effects of PFOS on growth performance, organizational microstructure, activities of immune-related enzymes and expressions of immune-related genes in male zebrafish (Danio rerio) exposed to different concentrations of 0, 0.02, 0.04 and 0.08 mg/L of PFOS for 7, 14, and 21 days or cotreatment with PFOS and PDTC to investigate the effects of PFOS on immune system and the potential toxic mechanisms caused by PFOS. The results indicated that PFOS accumulated in livers after exposure, and remarkably elevations were found in three exposure groups compared with the control group at three stages. The growth of the adult zebrafish in the experiments was significantly inhibited, the microstructures of liver were serious damaged. The ROS levels were remarkably increased. The activities of ACP, AKP, and lysozyme were obviously decreased, while the activities of MPO and NF-κB were significantly increased. The expressions of immune-related mRNA were significantly affected. After co-treatment with PFOS and PDTC, the growth inhibition, the morphological damage, the ROS induction, and the expressions of immune-related mRNA were reversed. Taken together, the results indicated that PFOS can significantly inhibit the growth, disturb the immune system by changing the normal structure of liver, the activities of immune-related enzymes, and a series of gene transcriptions involved in immune regulation in liver of male zebrafish. PFOS-induced pro-inflammatory effect of hepatocytes was observed, and the involvement of NF-κB signaling pathway was participated in its action mechanism. These findings provide further evidence that PFOS interferes with the immune regulation of liver of male zebrafish under in vivo conditions.
- Subjects :
- 0301 basic medicine
Fish Proteins
Male
Danio
Aquatic Science
03 medical and health sciences
chemistry.chemical_compound
Random Allocation
Immune system
In vivo
Thiocarbamates
Environmental Chemistry
Animals
Zebrafish
Messenger RNA
Fluorocarbons
biology
Dose-Response Relationship, Drug
NF-kappa B
04 agricultural and veterinary sciences
General Medicine
biology.organism_classification
Cell biology
Perfluorooctane
030104 developmental biology
chemistry
Alkanesulfonic Acids
Liver
040102 fisheries
0401 agriculture, forestry, and fisheries
Signal transduction
Growth inhibition
Reactive Oxygen Species
Water Pollutants, Chemical
Signal Transduction
Subjects
Details
- ISSN :
- 10959947
- Volume :
- 91
- Database :
- OpenAIRE
- Journal :
- Fishshellfish immunology
- Accession number :
- edsair.doi.dedup.....7b45c01f21fc5eb70e9ef1c35fc6675c