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Levels of Urinary Biomarkers of Oxidatively Generated Damage to DNA and RNA in Different Groups of Workers Compared to General Population
- Source :
- International Journal of Environmental Research and Public Health, Vol 16, Iss 16, p 2995 (2019), International Journal of Environmental Research and Public Health, Volume 16, Issue 16
- Publication Year :
- 2019
- Publisher :
- MDPI AG, 2019.
-
Abstract
- (1) Background: The products of guanine oxidation in DNA and RNA excreted in urine are 8-oxo-7,8-dihydroguanine (8-oxoGua), 8-oxo-7,8-dihydroguanosine (8-oxoGuo), and 8-oxo-7,8-dihydro-2&rsquo<br />deoxyguanosine (8-oxodGuo). Despite intra and inter-individual variability, it is possible to identify situations that significantly increase the levels of these compounds when comparing urinary concentrations of some workers to those of the general population. (2) Methods: urines from gasoline pump attendants (58 from Saudi Arabia and 102 from Italy), 24 workers of a fiberglass reinforced plastics plant, 17 painters and 6 divers were analyzed by HPLC/MS-MS. To test the individual variability, two subjects provided daily samples for one month, and 132 urine samples from the general population were analyzed. (3) Results: We summarized the results for each biomarker, and found the following were statistically higher than in the general population: 8-oxoGua in fiberglass and Italian gasoline workers<br />8-oxodGuo in fiberglass and both Saudi Arabian and Italian gasoline workers<br />8-oxoGuo in fiberglass workers, both Saudi Arabian and Italian gasoline workers, and painters after the working shift. (4) Conclusions: these results confirm that both 8-oxodGuo and 8-oxoGuo are valuable biomarkers for occupational exposures to dangerous chemicals and seem to suggest that 8-oxoGuo, related to RNA oxidation, is a suitable biomarker to evaluate short term, reversible effects of occupational exposures even within the health-based limit values.
- Subjects :
- Adult
Male
DNA and RNA oxidation
Health, Toxicology and Mutagenesis
Population
Saudi Arabia
Biological monitoring
Effect biomarkers
HPLC-MS/MS
Occupational exposure
Biomarkers
Chromatography, High Pressure Liquid
Female
Gasoline
Glass
Guanosine
Humans
Italy
Middle Aged
DNA Damage
Occupational Exposure
Oxidative Stress
RNA
lcsh:Medicine
Urine
010501 environmental sciences
01 natural sciences
Article
03 medical and health sciences
Environmental health
Medicine
education
030304 developmental biology
0105 earth and related environmental sciences
effect biomarkers
Chromatography
0303 health sciences
education.field_of_study
business.industry
lcsh:R
Public Health, Environmental and Occupational Health
occupational exposure
Urinary biomarkers
Hplc ms ms
biological monitoring
High Pressure Liquid
Biomarker (medicine)
business
Subjects
Details
- Language :
- English
- ISSN :
- 16604601
- Volume :
- 16
- Issue :
- 16
- Database :
- OpenAIRE
- Journal :
- International Journal of Environmental Research and Public Health
- Accession number :
- edsair.doi.dedup.....7b4252deec186e49f62c95579b0f73cb