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Integrating Target-Triggered Aptamer-Capped HRP@Metal-Organic Frameworks with a Colorimeter Readout for On-Site Sensitive Detection of Antibiotics
- Source :
- Analytical chemistry. 92(20)
- Publication Year :
- 2020
-
Abstract
- Colorimetric analytical strategies exhibit great promise in developing on-site detection methods for antibiotics, while substantial recent research efforts remain problematic due to dissatisfactory sensitivity. Taking this into account, we develop a novel colorimetric sensor for in-field detection of antibiotics by using aptamer (Apt)-capped and horseradish peroxidise (HRP)-embedded zeolitic metal azolate framework-7 (MAF-7) (Apt/HRP@MAF-7) as target recognition and signal transduction, respectively. With the substrate 3,3',5,5'-tetramethylbenzidine (TMB)-impregnated chip attached on the lid, the assay can be conveniently operated in a tube and reliably quantified by a handheld colorimeter. Hydrophilic MAF-7 can not only prevent HRP aggregation but also enhance HRP activity, which would benefit its detection sensitivity. Besides, the catalytic activity of HRP@MAF-7 can be sealed through assembling with Apt and controllably released based on the bioresponsivity via forming target-Apt complexes. Consequently, a significant color signal can be observed owing to the oxidation of colorless TMB to its blue-green oxidized form oxTMB. As a proof-of-concept, portable detection of streptomycin was favorably achieved with excellent sensitivity, which is superior to most reported methods and commercial kits. The developed strategy affords a new design pattern for developing on-site antibiotics assays and immensely extends the application of enzyme embedded metal-organic framework composites.
- Subjects :
- Aptamer
Biosensing Techniques
010402 general chemistry
01 natural sciences
Sensitivity and Specificity
Catalysis
Analytical Chemistry
Colorimetric sensor
Limit of Detection
Colorimetry
Coloring Agents
Horseradish Peroxidase
Metal-Organic Frameworks
Detection limit
Color signal
Chemistry
Benzidines
010401 analytical chemistry
Colorimeter
food and beverages
Substrate (chemistry)
Aptamers, Nucleotide
Enzymes, Immobilized
Combinatorial chemistry
0104 chemical sciences
Anti-Bacterial Agents
Streptomycin
Metal-organic framework
Oxidation-Reduction
Subjects
Details
- ISSN :
- 15206882
- Volume :
- 92
- Issue :
- 20
- Database :
- OpenAIRE
- Journal :
- Analytical chemistry
- Accession number :
- edsair.doi.dedup.....7b3a98f92baa39a72bfb34b8b6370f43