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Distribution, location, and transcriptional profile of Peyer's patch conventional DC subsets at steady state and under TLR7 ligand stimulation
- Source :
- Mucosal Immunology, 10(6), 1412-1430. Nature Publishing Group, Bonnardel, J, Da Silva, C, Wagner, C, Bonifay, R, Chasson, L, Masse, M, Pollet, E, Dalod, M, Gorvel, J P & Lelouard, H 2017, ' Distribution, location, and transcriptional profile of Peyer's patch conventional DC subsets at steady state and under TLR7 ligand stimulation ', Mucosal Immunology, vol. 10, no. 6, pp. 1412-1430 . https://doi.org/10.1038/mi.2017.30, Mucosal Immunology, Mucosal Immunology, Nature Pub. Group, 2017, 10 (6), pp.1412-1430. ⟨10.1038/mi.2017.30⟩, Mucosal Immunology, 2017, 10 (6), pp.1412-1430. ⟨10.1038/mi.2017.30⟩
- Publication Year :
- 2017
-
Abstract
- The initiation of the mucosal immune response in Peyer's patch (PP) relies on the sampling, processing, and efficient presentation of foreign antigens by dendritic cells (DCs). Among PP DCs, CD11b + conventional DCs (cDCs) and lysozyme-expressing DCs (LysoDCs) have distinct progenitors and functions but share many cell surface markers. This has previously led to confusion between these two subsets. In addition, another PP DC subset, termed double-negative (DN), remains poorly characterized. Here we show that both DN and CD11b + cDCs belong to a unique SIRPα + cDC subset. At steady state, cDCs and TIM-4 + macrophages are mainly located in T-cell zones, i.e., interfollicular regions, whereas a majority of subepithelial phagocytes are monocyte-derived cells, namely, LysoDCs and TIM-4 ' macrophages. Finally, oral administration of a Toll-like receptor 7 ligand induces at least three TNF-dependent events: (i) migration of dome-associated villus cDCs in interfollicular regions, (ii) increase of CD8α + interfollicular cDC number, and (iii) activation of both CD11b + and CD8α + interfollicular cDCs. The latter is marked by a genetic reprograming leading to the upregulation of type I interferon-stimulated and of both immuno-stimulatory and -inhibitory gene expression.
- Subjects :
- 0301 basic medicine
Cellular differentiation
Immunology
Antigen presentation
Mice, Transgenic
Biology
03 medical and health sciences
Mice
Peyer's Patches
Immune system
Downregulation and upregulation
medicine
Immunology and Allergy
Animals
Receptors, Immunologic
Receptor
ComputingMilieux_MISCELLANEOUS
Cells, Cultured
Antigen Presentation
CD11b Antigen
Membrane Glycoproteins
Tumor Necrosis Factor-alpha
Macrophages
Imidazoles
Peyer's patch
Membrane Proteins
Cell Differentiation
TLR7
Dendritic Cells
Cell biology
Mice, Inbred C57BL
030104 developmental biology
medicine.anatomical_structure
Toll-Like Receptor 7
[SDV.IMM]Life Sciences [q-bio]/Immunology
Tumor necrosis factor alpha
Muramidase
Transcriptome
Subjects
Details
- Language :
- English
- ISSN :
- 19330219 and 19353456
- Database :
- OpenAIRE
- Journal :
- Mucosal Immunology, 10(6), 1412-1430. Nature Publishing Group, Bonnardel, J, Da Silva, C, Wagner, C, Bonifay, R, Chasson, L, Masse, M, Pollet, E, Dalod, M, Gorvel, J P & Lelouard, H 2017, ' Distribution, location, and transcriptional profile of Peyer's patch conventional DC subsets at steady state and under TLR7 ligand stimulation ', Mucosal Immunology, vol. 10, no. 6, pp. 1412-1430 . https://doi.org/10.1038/mi.2017.30, Mucosal Immunology, Mucosal Immunology, Nature Pub. Group, 2017, 10 (6), pp.1412-1430. ⟨10.1038/mi.2017.30⟩, Mucosal Immunology, 2017, 10 (6), pp.1412-1430. ⟨10.1038/mi.2017.30⟩
- Accession number :
- edsair.doi.dedup.....7b25ba35b070e469312dbecc66f3087c