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Quercetin protects the retina by reducing apoptosis due to ischemia-reperfusion injury in a rat model
- Source :
- Arquivos Brasileiros de Oftalmologia v.78 n.2 2015, Arquivos brasileiros de oftalmologia, Conselho Brasileiro de Oftalmologia (CBO), instacron:CBO, Arquivos Brasileiros de Oftalmologia, Volume: 78, Issue: 2, Pages: 100-104, Published: APR 2015, Arquivos Brasileiros de Oftalmologia, Vol 78, Iss 2, Pp 100-104 (2015), Scopus-Elsevier
- Publication Year :
- 2015
- Publisher :
- Conselho Brasileiro de Oftalmologia, 2015.
-
Abstract
- Purpose: This study aimed to investigate the effect of quercetin on apoptotic cell death induced by ischemia-reperfusion (I/R) injury in the rat retina. Methods: Twenty-four rats were divided into four equal groups: control, ischemic, solvent, and quercetin. I/R injury was achieved by elevating the intraocular pressure above the perfusion pressure. Intraperitoneal injections of 20 mg/kg of quercetin and dimethyl sulfoxide (DMSO) were performed in the quercetin and solvent groups, respectively, immediately prior to I/R injury, and the researchers allowed for the retinas to be reperfused. Forty-eight hours after injury, the thicknesses of the retinal ganglion cell layer (RGCL), inner nuclear layer (INL), inner plexiform layer (IPL), outer plexiform layer (OPL), and outer nuclear layer (ONL) were measured in all groups. Moreover, the numbers of terminal deoxynucleotidyl transferase dUTP nick-end-labeled [TUNEL (+)] cells and caspase-3 (+) cells in both INL and ONL were evaluated in all groups. Results: The administration of quercetin was found to reduce the thinning of all retinal layers. The mean thickness of INL in the quercetin and ischemic groups was 21 ± 5.6 µm and 16 ± 6.4 µm, respectively (P
- Subjects :
- Male
Cell Count
Apoptosis
Antioxidants
chemistry.chemical_compound
Traumatismo por reperfusão
lcsh:Ophthalmology
Ischemia
Quercetina
heterocyclic compounds
Retina/efeitos de drogas
Ratos
TUNEL assay
Caspase 3
General Medicine
Immunohistochemistry
Reperfusion injury
medicine.anatomical_structure
Treatment Outcome
Reperfusion Injury
Retina/drug effects
Inner nuclear layer
Modelos animais de doenças
Quercetin
medicine.medical_specialty
Outer plexiform layer
Disease models
Retina
Andrology
Retinal Diseases
medicine
In Situ Nick-End Labeling
Animals
Dimethyl Sulfoxide
Rats, Wistar
Outer nuclear layer
Intraocular Pressure
business.industry
Apoptose
Reproducibility of Results
Retinal
Inner plexiform layer
Surgery
Rats
Isquemia
Ophthalmology
Disease Models, Animal
chemistry
lcsh:RE1-994
Solvents
sense organs
business
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Arquivos Brasileiros de Oftalmologia v.78 n.2 2015, Arquivos brasileiros de oftalmologia, Conselho Brasileiro de Oftalmologia (CBO), instacron:CBO, Arquivos Brasileiros de Oftalmologia, Volume: 78, Issue: 2, Pages: 100-104, Published: APR 2015, Arquivos Brasileiros de Oftalmologia, Vol 78, Iss 2, Pp 100-104 (2015), Scopus-Elsevier
- Accession number :
- edsair.doi.dedup.....7b1e51803648eb93b7d9146c9a70bcbd