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Quercetin protects the retina by reducing apoptosis due to ischemia-reperfusion injury in a rat model

Authors :
Ismail Ersan
Tufan Hasan Ali
Baran Gencer
Turan Karaca
Sedat Arikan
Selçuk Kara
İhsan Karaboğa
Source :
Arquivos Brasileiros de Oftalmologia v.78 n.2 2015, Arquivos brasileiros de oftalmologia, Conselho Brasileiro de Oftalmologia (CBO), instacron:CBO, Arquivos Brasileiros de Oftalmologia, Volume: 78, Issue: 2, Pages: 100-104, Published: APR 2015, Arquivos Brasileiros de Oftalmologia, Vol 78, Iss 2, Pp 100-104 (2015), Scopus-Elsevier
Publication Year :
2015
Publisher :
Conselho Brasileiro de Oftalmologia, 2015.

Abstract

Purpose: This study aimed to investigate the effect of quercetin on apoptotic cell death induced by ischemia-reperfusion (I/R) injury in the rat retina. Methods: Twenty-four rats were divided into four equal groups: control, ischemic, solvent, and quercetin. I/R injury was achieved by elevating the intraocular pressure above the perfusion pressure. Intraperitoneal injections of 20 mg/kg of quercetin and dimethyl sulfoxide (DMSO) were performed in the quercetin and solvent groups, respectively, immediately prior to I/R injury, and the researchers allowed for the retinas to be reperfused. Forty-eight hours after injury, the thicknesses of the retinal ganglion cell layer (RGCL), inner nuclear layer (INL), inner plexiform layer (IPL), outer plexiform layer (OPL), and outer nuclear layer (ONL) were measured in all groups. Moreover, the numbers of terminal deoxynucleotidyl transferase dUTP nick-end-labeled [TUNEL (+)] cells and caspase-3 (+) cells in both INL and ONL were evaluated in all groups. Results: The administration of quercetin was found to reduce the thinning of all retinal layers. The mean thickness of INL in the quercetin and ischemic groups was 21 ± 5.6 µm and 16 ± 6.4 µm, respectively (P

Details

Language :
English
Database :
OpenAIRE
Journal :
Arquivos Brasileiros de Oftalmologia v.78 n.2 2015, Arquivos brasileiros de oftalmologia, Conselho Brasileiro de Oftalmologia (CBO), instacron:CBO, Arquivos Brasileiros de Oftalmologia, Volume: 78, Issue: 2, Pages: 100-104, Published: APR 2015, Arquivos Brasileiros de Oftalmologia, Vol 78, Iss 2, Pp 100-104 (2015), Scopus-Elsevier
Accession number :
edsair.doi.dedup.....7b1e51803648eb93b7d9146c9a70bcbd