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Insulin induces calcium signals in the nucleus of rat hepatocytes
- Source :
- Hepatology. 48:1621-1631
- Publication Year :
- 2008
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2008.
-
Abstract
- Insulin is an hepatic mitogen that promotes liver regeneration. Actions of insulin are mediated by the insulin receptor, which is a receptor tyrosine kinase. It is currently thought that signaling via the insulin receptor occurs at the plasma membrane, where it binds to insulin. Here we report that insulin induces calcium oscillations in isolated rat hepatocytes, and that these calcium signals depend upon activation of phospholipase C and the inositol 1,4,5-trisphosphate receptor, but not upon extracellular calcium. Furthermore, insulin-induced calcium signals occur in the nucleus, and are temporally associated with selective depletion of nuclear phosphatidylinositol bisphosphate and translocation of the insulin receptor to the nucleus. These findings suggest that the insulin receptor translocates to the nucleus to initiate nuclear, inositol 1,4,5-trisphosphate-mediated calcium signals in rat hepatocytes. This novel signaling mechanism may be responsible for insulin's effects on liver growth and regeneration.
- Subjects :
- Male
Phosphatidylinositol 4,5-Diphosphate
medicine.medical_specialty
medicine.medical_treatment
Inositol 1,4,5-Trisphosphate
Rats, Sprague-Dawley
Internal medicine
Insulin receptor substrate
medicine
Animals
Insulin
TRPM3
Phosphorylation
Insulin-like growth factor 1 receptor
Cell Nucleus
Microscopy, Confocal
Dose-Response Relationship, Drug
Hepatology
biology
GRB10
Receptor, Insulin
IRS2
Rats
Insulin receptor
Endocrinology
Hepatocytes
biology.protein
Calcium
Signal transduction
Signal Transduction
Subjects
Details
- ISSN :
- 02709139
- Volume :
- 48
- Database :
- OpenAIRE
- Journal :
- Hepatology
- Accession number :
- edsair.doi.dedup.....7b0a32a6b79cd293587ecacbe2bd0c7d