Effect of a Stellate Ganglion Block on Acute Lung Injury in Septic Rats

A stellate ganglion block (SGB) is a clinical sympathetic block which can inhibit the body systemic inflammatory response. However, whether and how SGB can attenuate the sepsis-induced acute lung injury remains unclear. Here, we evaluated the effect of SGB on sepsis-induced acute lung injury in rats. Ninety healthy Sprague Dawley (SD) male rats were divided into three groups: the sham operation group (S group), sepsis group (Sep group), and SGB group. The sepsis model rats were produced by cecum ligation and puncture (CLP), and blood samples were taken from the abdominal aorta of the rats at different time points for evaluating the concentration of TNF-α, IL-6, and IL-10 by enzyme-linked immunosorbent assay (ELISA). The rats were sacrificed, and lungs were collected to measure the wet/dry (W/D) lung tissue weight ratio, score the lung tissue pathological damage by microscopic examination, determine the myeloperoxidase (MPO) activity by spectrophotometry, and measure nuclear factor-kappa B (NF-κB) p65 expression by Western blot. The concentration of serum TNF-α, IL-6, and IL-10, lung tissue W/D ratio, pathological injury score, MPO activity, and expression of NF-κB p65 were higher in the Sep group compared with the S group at T1-4. Furthermore, the concentration of serum TNF-α and IL-6, lung tissue W/D ratio, pathological damage score, MPO activity, and expression of NF-κB p65 were reduced and the concentration of IL-10 was increased in the SGB group compared with the Sep group at T1-4. The successful sepsis model rats were induced by CLP, and SGB attenuated the sepsis-induced acute lung injury in rats.