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Long and branched polyamines are required for maintenance of the ribosome, tRNA(His) and tRNA(Tyr) in Thermus thermophilus cells at high temperatures
- Source :
- Genes to Cells: Devoted to Molecular & Cellular Mechanisms, Genes to Cells: Devoted to Molecular & Cellular Mechanisms, 2017, ⟨10.1111/gtc.12502⟩
- Publication Year :
- 2017
- Publisher :
- HAL CCSD, 2017.
-
Abstract
- Thermus thermophilus is an extremely thermophilic eubacterium that produces various polyamines. Aminopropylagmatine ureohydrolase (SpeB) and SAM decarboxylase-like protein 1 (SpeD1) are involved in the biosynthesis of spermidine from arginine. Because long and branched polyamines in T. thermophilus are synthesized from spermidine, the speB and speD1 gene-deleted strains (ΔspeB and ΔspeD1, respectively) cannot synthesize long and branched polyamines. Although neither strain grew at high temperatures (\textgreater75°C) in minimal medium, both strains survived at 80°C when they were cultured at 70°C until the mid-log phase and then shifted to 80°C. We therefore prepared the ΔspeB and ΔspeD1 cells using this culture method. Microscopic analysis showed that both strains can survive for 10 h after the temperature shift. Although the modification levels of 2'-O-methylguanosine at position 18, N(7) -methylguanosine at position 46, 5-methyluridine at position 54 and N(1) -methyladenosine at position 58 in the class I tRNA from both strains were normal, amounts of tRNA(Tyr) , tRNA(His) , rRNAs and 70S ribosomes were decreased after the temperature shift. Furthermore, in vivo protein synthesis in both strains was completely lost 10 h after the temperature shift. Thus, long and branched polyamines are required for at least the maintenance of 70S ribosome and some tRNA species at high temperatures.
- Subjects :
- 0301 basic medicine
030102 biochemistry & molecular biology
Arginine
biology
Thermophile
[SDV]Life Sciences [q-bio]
Cell Biology
Thermus thermophilus
biology.organism_classification
Ribosome
Spermidine
03 medical and health sciences
chemistry.chemical_compound
030104 developmental biology
chemistry
Biosynthesis
Biochemistry
Transfer RNA
Genetics
Protein biosynthesis
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Genes to Cells: Devoted to Molecular & Cellular Mechanisms, Genes to Cells: Devoted to Molecular & Cellular Mechanisms, 2017, ⟨10.1111/gtc.12502⟩
- Accession number :
- edsair.doi.dedup.....7ae88b3b143d010b0e9ab5515674b43a