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Histologic Observation and Significance of Sympathetic Nerve Fiber Distribution on Human Cervical Ligamentum Flavum
- Source :
- Orthopaedic Surgery
- Publication Year :
- 2020
- Publisher :
- Wiley, 2020.
-
Abstract
- Objective To study the distribution of sympathetic nerves of the ligamentum flavum (LF), confirm its existence by histological observation and nuclear magnetic resonance spectroscopy, and analyze the relationship between sympathetic nerve fibers and the biomechanical structure of the LF. Methods Randomly controlled scientific research selected 15 cases of posterior surgery in the affiliated hospital of Qingdao University from January 2013 to December 2019. The average age was 67.5 ± 14.5 years old, eight males and seven females. The LF specimens (completely separated fresh tissue) of different segments (C3‐7) were taken during the operation. Two pages of LF specimens on the left and right sides of the same segment are randomly allocated by the pairing method for formalin fixation and cryopreservation in liquid nitrogen. LF specimens extracted from seven other adult cadaver specimens (average age at death of about 56.8 ± 4.0 years, three males and four females) were used as a control group; together with formalin‐ fixed specimens obtained during surgery, 3D slices were given layer by layer. The distribution of sympathetic nerves in different parts of the LF was analyzed by glyoxylic acid‐induced biological monoamine fluorescent technique (SPG) and hematoxylin–eosin (HE) staining. Fifteen liquid nitrogen storage specimens were divided into the back of the LF and the spinal canal through frozen sections, and were analyzed by nuclear magnetic resonance spectroscopy‐hydrogen spectrum (1H ‐NMR) for neurotransmitters and neurometabolites. Results There were type C sympathetic nerve fibers in the LF, which were divided into linear shape (α) and wave shape (β). Experimental group (χ2 = 1.705, P > 0.05) and control group (χ2 = 0.879, P > 0.05) can detect no difference in fluorescence units. Nerve fiber transmitter metabolites choline (Cho), creator (Cr), γ‐aminobutyric acid (GABA) also indicate that the sympathetic nerve is present in the LF. LF sympathetic nerve fibers were mainly distributed in the proximal spinal canal surface, nerve fibers on the medial belt (area II) were fewer than the lateral belt (area I) (W = 210, P<br />Sympathetic nerve fibers distributed on ligamentum flavum. Their distribution may be correlated with histological and mechanical characteristics of ligamentum flavum. It may also be the anatomical basis of cervical vertigo.
- Subjects :
- Male
Magnetic Resonance Spectroscopy
Vertebral artery dissection
Nerve fiber
03 medical and health sciences
Nerve Fibers
0302 clinical medicine
Cadaver
medicine
Cervical spondylosis
Humans
Orthopedics and Sports Medicine
Spinal canal
Aged
Aged, 80 and over
Cervical instability
030222 orthopedics
Frozen section procedure
Clinical Article
business.industry
Anatomy
Middle Aged
Cervical vertigo
medicine.disease
Staining
Ligamentum Flavum
Monoamine neurotransmitter
medicine.anatomical_structure
Clinical Articles
Cervical Vertebrae
Sympathetic nerve fiber
Female
Surgery
business
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 17577861 and 17577853
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Orthopaedic Surgery
- Accession number :
- edsair.doi.dedup.....7adf821de1b3184df58842357454a958