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PECTIN ACETYLESTERASE9 Affects the Transcriptome and Metabolome and Delays Aphid Feeding
- Source :
- Plant Physiology 181 (2019) 4, Plant Physiology, 181(4), 1704-1720, Plant Physiology, Plant Physiology, American Society of Plant Biologists, 2019, 181 (4), pp.1704-1720. ⟨10.1104/pp.19.00635⟩
- Publication Year :
- 2019
-
Abstract
- International audience; The plant cell wall plays an important role in damage-associated molecular pattern-induced resistance to pathogens and herbivorous insects. Our current understanding of cell wall-mediated resistance is largely based on the degree of pectin methylesterification. However, little is known about the role of pectin acetylesterification in plant immunity. This study describes how one pectin-modifying enzyme, PECTIN ACETYLESTERASE 9 (PAE9), affects the Arabidopsis (Arabidopsis thaliana) transcriptome, secondary metabolome, and aphid performance. Electro-penetration graphs showed that Myzus persicae aphids established phloem feeding earlier on pae9 mutants. Whole-genome transcriptome analysis revealed a set of 56 differentially expressed genes (DEGs) between uninfested pae9-2 mutants and wild-type plants. The majority of the DEGs were enriched for biotic stress responses and down-regulated in the pae9-2 mutant, including PAD3 and IGMT2, involved in camalexin and indole glucosinolate biosynthesis, respectively. Relative quantification of more than 100 secondary metabolites revealed decreased levels of several compounds, including camalexin and oxylipins, in two independent pae9 mutants. In addition, absolute quantification of phytohormones showed that jasmonic acid (JA), jasmonoyl-Ile, salicylic acid, abscisic acid, and indole-3-acetic acid were compromised due to PAE9 loss of function. After aphid infestation, however, pae9 mutants increased their levels of camalexin, glucosinolates, and JA, and no long-term effects were observed on aphid fitness. Overall, these data show that PAE9 is required for constitutive up-regulation of defense-related compounds, but that it is not required for aphid-induced defenses. The signatures of phenolic antioxidants, phytoprostanes, and oxidative stress-related transcripts indicate that the processes underlying PAE9 activity involve oxidation-reduction reactions.
- Subjects :
- 0106 biological sciences
Indoles
Physiology
Resistance
Arabidopsis
Secondary Metabolism
Plant Science
01 natural sciences
Myzus-persicae
Transcriptome
chemistry.chemical_compound
Arabidopsis-thaliana
Plant Growth Regulators
Gene Expression Regulation, Plant
Genes, Regulator
Camalexin
Arabidopsis thaliana
Gene Regulatory Networks
Laboratory of Entomology
Polysaccharide
Abscisic acid
Research Articles
Defense Responses
Phytoalexin Accumulation
Jasmonic acid
food and beverages
Green Peach Aphid
Biosystematiek
Biochemistry
Metabolome
Plant-cell Wall
Glucosinolates
Down-Regulation
Biology
Stress-response
Genetics
Animals
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
Life Science
Herbivory
Oxylipins
Arabidopsis Proteins
Biotic stress
biology.organism_classification
Laboratorium voor Entomologie
Oxidative Stress
Thiazoles
chemistry
Aphids
Mutation
Host-pathogen Interactions
Biosystematics
Acetylesterase
Transcription Factors
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 00320889 and 15322548
- Volume :
- 181
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Plant Physiology
- Accession number :
- edsair.doi.dedup.....7abae6a28e0eb434cd16e03e988ae7ac