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Application of a novel phage vB_SalS-LPSTLL for the biological control of Salmonella in foods
- Source :
- Food research international (Ottawa, Ont.). 147
- Publication Year :
- 2021
-
Abstract
- Salmonella is one of the most common foodborne pathogens around the world. Phages are envisioned as a new strategy to control foodborne pathogenic bacteria and food safety. A Salmonella specific lytic phage vB_SalS-LPSTLL (LPSTLL) was selected for food applications on the basis of lytic range, lytic efficiency, functional stability and characteristics. Phage LPSTLL was able to lyse 11 Salmonella serotypes, which represents the broadest range reported Salmonella phages, and was able to suppress the growth of Salmonella enterica in liquid culture over nine hours. LPSTLL exhibited rapid reproductive activity with a short latent period and a large burst size in one-step growth experiment. LPSTLL remained active over a pH range of 3.0 to 12.0, and at incubation temperatures up to 60 °C for 60 min, indicating wide applicability for food processing and storage. Significant reductions of viable Salmonella were observed in diverse foods (milk, apple juice, chicken and lettuce) with reductions up to 2.8 log CFU/mL recorded for milk. Sensory evaluation indicated that treatment with phage LPSTLL did not alter the visual or tactile quality of food matrices. Genome analysis of LPSTLL indicated the absence of any virulence or antimicrobial resistance genes. Genomic comparisons suggest phage LPSTLL constitutes a novel member of a new genus, the LPSTLLvirus with the potential for Salmonella biocontrol in the food industry.
- Subjects :
- Serotype
Salmonella
Food Safety
Food industry
030309 nutrition & dietetics
Virulence
Biology
medicine.disease_cause
Microbiology
03 medical and health sciences
0404 agricultural biotechnology
medicine
Bacteriophages
0303 health sciences
business.industry
Pathogenic bacteria
04 agricultural and veterinary sciences
Food safety
biology.organism_classification
040401 food science
Lytic cycle
Salmonella enterica
Salts
business
Salmonella Phages
Food Science
Subjects
Details
- ISSN :
- 18737145
- Volume :
- 147
- Database :
- OpenAIRE
- Journal :
- Food research international (Ottawa, Ont.)
- Accession number :
- edsair.doi.dedup.....7ab7dd6ca2b127f69cf87c0cbaed3f28