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Counter‐flow gradient electrophoresis for focusing and elution
- Source :
- ELECTROPHORESIS.
- Publication Year :
- 2018
- Publisher :
- Wiley, 2018.
-
Abstract
- Counterflow gradient electrofocusing uses the bulk flow of a liquid solution to counterbalance the electrophoretic migration of an analyte. When either the bulk velocity or the electrophoretic velocity of the analyte is made to vary across the length of the channel, there exists a unique zero-velocity point for the analyte. This focusing method enables simultaneous separation and concentration of different analytes. The high resolution and sensitivity achieved are similar to that of isoelectric focusing, which separates analytes based on their isoelectric points, but the key difference is that analytes will instead focus based on their electrophoretic mobility. Dynamically changing the applied voltage or the counterflow rate over time will shift the zero-velocity point, and therefore allows the focused analytes to pass through a fixed detection point, or elute from the separation channel. Throughout the review, a number of different counterflow gradient techniques will be discussed, along with their recent advancements and potential applications.
- Subjects :
- Electrophoresis
Analyte
Materials science
010405 organic chemistry
Elution
Isoelectric focusing
Clinical Biochemistry
Analytical chemistry
Proteins
Equipment Design
010402 general chemistry
01 natural sciences
Biochemistry
0104 chemical sciences
Analytical Chemistry
Capillary electrophoresis
Isoelectric point
Bulk velocity
Countercurrent Distribution
Electrodes
Voltage
Subjects
Details
- ISSN :
- 15222683 and 01730835
- Database :
- OpenAIRE
- Journal :
- ELECTROPHORESIS
- Accession number :
- edsair.doi.dedup.....7ab3c64c15d8b3834496d015966c741c