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Reduced expression of galectin-1 and galectin-9 by leucocytes in asthma patients
- Source :
- Europe PubMed Central
- Publication Year :
- 2012
- Publisher :
- Oxford University Press (OUP), 2012.
-
Abstract
- Summary Accumulating evidence shows that galectins play roles in the initiation and resolution phases of inflammatory responses by promoting anti- or proinflammatory effects. This study investigated the presence of three members of the galectin family (galectin-1, -3 and -9) in induced sputum samples of asthma patients, as well as their possible implication in the immunopathogenesis of human asthma. Levels of interleukin (IL)-5, IL-13, and galectins were determined in leucocytes isolated from induced sputum samples by reverse transcription–polymerase chain reaction (RT–PCR) immunofluorescence and flow cytometry. High levels of IL-5 and IL-13 mRNA were detected in sputum cells from asthma patients. In parallel, immunoregulatory proteins galectin-1 and galectin-9 showed a reduced expression on macrophages from sputum samples compared with cells from healthy donors. In-vitro immunoassays showed that galectin-1 and galectin-9, but not galectin-3, are able to induce the production of IL-10 by peripheral blood mononuclear cells from healthy donors. These findings indicate that macrophages from sputum samples of asthma patients express low levels of galectin-1 and galectin-9, favouring the exacerbated immune response observed in this disease.
- Subjects :
- Adult
Male
animal structures
Galectin 1
Galectin 3
Immunology
Biology
Immunofluorescence
Peripheral blood mononuclear cell
Proinflammatory cytokine
Young Adult
Th2 Cells
Immune system
Leukocytes
otorhinolaryngologic diseases
medicine
Humans
Immunology and Allergy
Aged
Galectin
medicine.diagnostic_test
Macrophages
Sputum
Interleukin
Original Articles
Middle Aged
Asthma
respiratory tract diseases
Respiratory Function Tests
stomatognathic diseases
Gene Expression Regulation
Case-Control Studies
Galectin-1
Cytokines
Female
medicine.symptom
Subjects
Details
- ISSN :
- 13652249 and 00099104
- Volume :
- 170
- Database :
- OpenAIRE
- Journal :
- Clinical and Experimental Immunology
- Accession number :
- edsair.doi.dedup.....7aaa8c2e8af84d2b5ae44dee2f2f600d