Marker assisted backcross to introgress late leaf spot and rust resistance in groundnut (Arachis hypogaea L.)

Groundnut is affected by a variety of abiotic and biotic stressors, including late leaf spot and rust, which cause significant economic loss. In this study, QTL for resistance to late leaf spot and rust from donor variety GPBD 4 were incorporated into a popular groundnut variety (ICGV 00350) through marker assisted backcross (MABC) breeding.Eight foreground SSR markers [AhXII (GM1009, GM1573 and Seq8D09) and AhXV (GM1536, GM2009, GM2079, GM2301 and IPAHM103)] linked with disease resistant QTLs were utilized in this study. A set of 217 SSR markers spanning the whole groundnut genome were employed for background analysis. Three backcrosses with recurrent parent and selfing were followed in the cross ICGV 00350 × GPBD 4. Background analysis was carried out in BCFive advanced backcross lines in BC