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Determination of carotenoids and all-trans-retinol in fish eggs by liquid chromatography–electrospray ionization–tandem mass spectrometry
- Source :
- Great Lakes Institute for Environmental Research Publications
- Publication Year :
- 2005
- Publisher :
- Elsevier BV, 2005.
-
Abstract
- A novel method was developed for the combined determination of carotenoids and retinoids in fish eggs, which incorporates prior analyte isolation using liquid-liquid partitioning to minimize analyte degradation, and fraction analysis using high-performance liquid chromatography-electrospray (positive)-quadrupole mass spectrometry (LC-ESI(+)-MS; SIM or MRM modes). Eggs from Chinook salmon (Oncorhynchus tshawytscha) were used as the model fish egg matrix. The methodology was assessed and validated for beta-carotene, lutein, zeaxanthin, and beta-cryptoxanthin (molecular ion radicals [M](+)), canthaxanthin and astaxanthin ([M+Na](+) adducts) and all-trans-retinol ([(M+H)-H(2)O](+)). Using replicate egg samples (n=5) spiked with beta-cryptoxanthin and beta-carotene before and after extraction, matrix-sourced ESI(+) enhancement was observed as evidenced by comparable %matrix effect and %process efficiency values for beta-cryptoxanthin and beta-carotene of 114-119%. In aquaculture-raised eggs from adult Chinook salmon astaxanthin, all-trans-retinol, lutein and canthaxanthin were identified and determined at concentrations of 4.12, 1.06, 0.12 and 0.45 microg/g (egg wet weight), respectively. To our knowledge, this is the first report on a method for LC-MS determination of carotenoids and retinoids in a fish egg matrix, and the first carotenoid-specific determination in any fish egg sample.
- Subjects :
- Spectrometry, Mass, Electrospray Ionization
Lutein
Eggs
Clinical Biochemistry
Marine Biology
Xanthophylls
Mass spectrometry
Biochemistry
Analytical Chemistry
Matrix (chemical analysis)
Retinoids
Fish eggs
chemistry.chemical_compound
Salmon
Astaxanthin
Animals
Canthaxanthin
All trans retinol
Vitamin A
Biology
Carotenoid
Chromatography, High Pressure Liquid
Biochemistry, Biophysics, and Structural Biology
Ovum
chemistry.chemical_classification
Chromatography
Life Sciences
Biodiversity
Cell Biology
General Medicine
LC–electrospray–tandem (quadrupole) MS
beta Carotene
Carotenoids
Zeaxanthin
chemistry
Chinook salmon
Chickens
Subjects
Details
- ISSN :
- 15700232
- Volume :
- 816
- Database :
- OpenAIRE
- Journal :
- Journal of Chromatography B
- Accession number :
- edsair.doi.dedup.....7a5651ab7b93f128b2e1f8b2d5933626