Back to Search
Start Over
Expression of the high molecular weight glutenin 1Ay gene from Triticum urartu in barley
- Source :
- Transgenic Research. 28:225-235
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- In this study, we successfully expressed the active 1Ay subunit of Triticum urartu in barley by Agrobacterium-mediated transformation with a transformation efficiency of 19.9%. The results of SDS-PAGE revealed that the expressed proteins of 1Ay subunit were present at some grains of each of 46 original T0 plants, showing identical mobility to those of positive standards of T. urartu grain protein and bacteria expressional proteins. In the T2 generation, three homozygous lines, 2-28, 3-11, and 5-6, were identified. The results of scanning electron microscopy showed an increased amount of protein bodies in these transgenic lines. The main effects in the expression of the 1Ay subunits was a considerable increase in the glutenin content, but a decrease in the contents of gliadins while there were no effects in the contents of albumin, globulin and the total protein. We found that the gluten could not be washed out from the flour obtained from transgenic barley lines when using a Gluten index analyzer and a Farinograph indicating that the transgenic barley lines could not form dough. The lack of x-type HMW-GS and the reduction in number of subunit were inferred as the possible reasons for the inability to form gluten polymer.
- Subjects :
- chemistry.chemical_classification
Farinograph
Glutens
biology
Protein subunit
Flour
food and beverages
Hordeum
Plants, Genetically Modified
Gluten
Molecular Weight
Protein Subunits
Transformation (genetics)
Glutenin
Triticum urartu
chemistry
Biochemistry
Genetics
biology.protein
Animal Science and Zoology
Hordeum vulgare
Gliadin
Agronomy and Crop Science
Triticum
Biotechnology
Subjects
Details
- ISSN :
- 15739368 and 09628819
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- Transgenic Research
- Accession number :
- edsair.doi.dedup.....7a51fe1fc3014c67488577174d54e6c0