Back to Search
Start Over
Development of immunogenic colorectal cancer cell lines for vaccination: expression of CD80 (B7.1) is not sufficient to restore impaired primary T cell activation in vitro
- Source :
- European journal of cancer (Oxford, England : 1990). (13-14)
- Publication Year :
- 1995
-
Abstract
- The capacity of colorectal carcinoma and melanoma cell lines to induce primary versus effector T lymphocyte activation in vitro was investigated. Established epithelial tumour cell lines derived from colorectal carcinoma and melanoma did not activate a primary proliferative response of resting T lymphocytes in allogeneic mixed lymphocyte tumour cell cultures (MLTCs). In contrast, the same tumour cells were effectively lysed by preactivated cytolytic T cell clones. This demonstrates that tumour cells are impaired in inducing a primary immune response but are susceptible to effector immune responses. Attempts at improving primary T cell activation revealed that exogenous cytokines, including interferon-gamma (IFN-gamma), tumour necrosis factor-alpha (TNF-alpha) and interleukin-2 (IL-2), were not effective. Expression of CD80 (B7.1), by transfecting a CD80 cDNA into the melanoma cell line SkMel63, improved T cell proliferation considerably. In contrast, CD80 expression in two colorectal carcinoma cell lines (SW480, SW707) did not result in T cell activation. This was not due to lack of class II MHC expression on SW480 since coexpression of a HLA-DR3 alloantigen and CD80 had no effect. Our data suggest that de novo CD80 expression is not, in general, sufficient to improve primary T cell activation by human tumour cells.
- Subjects :
- Cancer Research
Lymphocyte
T cell
T-Lymphocytes
chemical and pharmacologic phenomena
Major histocompatibility complex
Lymphocyte Activation
Immune system
HLA-DR3 Antigen
Antigens, Neoplasm
medicine
Tumor Cells, Cultured
Cytotoxic T cell
Humans
Melanoma
biology
Gene Transfer Techniques
Cytolysis
medicine.anatomical_structure
Oncology
Genes
Cell culture
Immunology
Cancer research
biology.protein
B7-1 Antigen
Cytokines
Lymphocyte Culture Test, Mixed
Colorectal Neoplasms
CD80
Subjects
Details
- ISSN :
- 09598049
- Issue :
- 13-14
- Database :
- OpenAIRE
- Journal :
- European journal of cancer (Oxford, England : 1990)
- Accession number :
- edsair.doi.dedup.....7a506628a66d8191d8784b03027d1f4b