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Automated image analysis of cytokinesis-blocked micronuclei: an adapted protocol and a validated scoring procedure for biomonitoring
- Source :
- Mutagenesis. 24:85-93
- Publication Year :
- 2008
- Publisher :
- Oxford University Press (OUP), 2008.
-
Abstract
- Micronuclei (MN) frequencies in peripheral blood lymphocytes have been used worldwide as a biomarker of chromosomal damage for genotoxicity testing and biomonitoring studies. Automation of MN analysis would provide faster and more reliable results with minimizing subjective MN identification. We developed an automated facility for the scoring of the in vitro MN cytokinesis-block assay for biomonitoring on Giemsa-stained slides, fulfilling the following criteria: applicable to the cytokinesis-block micronucleus methodology, discriminating between mono-, bi- and polynucleated cells, MN scoring according to HUMN scoring criteria, false-negative MN rate
- Subjects :
- Adult
Computer science
Health, Toxicology and Mutagenesis
Binucleated cells
Scoring criteria
Toxicology
medicine.disease_cause
Biomonitoring
Image Processing, Computer-Assisted
Genetics
medicine
Humans
Lymphocytes
Micronuclei, Chromosome-Defective
Genetics (clinical)
automation
Cytokinesis
Protocol (science)
Micronucleus Tests
Reproducibility of Results
Peripheral blood
cytokinesis-blocked micronuclei
micronucleus assay
automated analysis
biomonitoring
Micronucleus test
Micronucleus
Biomarkers
Genotoxicity
DNA Damage
Environmental Monitoring
Biomedical engineering
Subjects
Details
- ISSN :
- 14643804 and 02678357
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- Mutagenesis
- Accession number :
- edsair.doi.dedup.....7a4b6e69c10a05694ca03a4cc0b43b55