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Surface display of hirame novirhabdovirus (HIRRV) G protein in Lactococcus lactis and its immune protection in flounder (Paralichthys olivaceus)
- Source :
- Microbial Cell Factories, Vol 18, Iss 1, Pp 1-13 (2019), Microbial Cell Factories
- Publication Year :
- 2019
- Publisher :
- BMC, 2019.
-
Abstract
- Background Hirame novirhabdovirus (HIRRV) can infect a wide range of marine and freshwater fish, causing huge economic losses to aquaculture industry. Vaccine development, especially oral vaccine, has become an effective and convenient way to control aquatic infectious diseases. HIRRV glycoprotein (G), an immunogenic viral protein is a potential vaccine candidate for prevention of the disease. Here, we aimed to construct a recombinant Lactococcus lactis strain expressing HIRRV-G on the cell surface as an oral vaccine to prevent HIRRV. Results Glycoprotein gene of HIRRV was successfully cloned and expressed in L. lactis NZ9000 in a surface-displayed form, yielding Ll:pSLC-G. An approximately 81 kDa recombinant G protein (containing LysM anchoring motif) was confirmed by SDS-PAGE, western blotting and mass spectrometry analysis. The surface-displayed G protein was also verified by immunofluorescence and flow cytometry assays. Furthermore, to evaluate the potential of Ll:pSLC-G as oral vaccine candidate, flounders were continuously fed with commercial diet pellets coated with 1.0 × 109 cfu/g of induced Ll:pSLC-G for 1 week. Four weeks later, booster vaccination was performed with the same procedure. Compared with the controls, Ll:pSLC-G elicited significantly higher levels of specific IgM against HIRRV in flounder gut mucus at the second week and in serum at the fourth week (p
- Subjects :
- 0106 biological sciences
G protein
HIRRV glycoprotein
lcsh:QR1-502
Bioengineering
Flounder
01 natural sciences
Applied Microbiology and Biotechnology
lcsh:Microbiology
law.invention
Flow cytometry
Microbiology
Novirhabdovirus
Fish Diseases
Viral Proteins
03 medical and health sciences
Immune system
law
010608 biotechnology
medicine
Oral vaccine
Animals
Cloning, Molecular
Immune protection
Glycoproteins
030304 developmental biology
chemistry.chemical_classification
Vaccines, Synthetic
0303 health sciences
biology
medicine.diagnostic_test
Research
Vaccination
Lactococcus lactis
biology.organism_classification
Surface display
Mucus
Recombinant Proteins
chemistry
Virus Diseases
Recombinant DNA
Genetic Engineering
Glycoprotein
Biotechnology
Subjects
Details
- Language :
- English
- ISSN :
- 14752859
- Volume :
- 18
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Microbial Cell Factories
- Accession number :
- edsair.doi.dedup.....7a3564bbdff47df5e99008f82f33b0ba