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CD24 and myosin light polypeptide 2 are involved in prevention of experimental autoimmune encephalomyelitis by myelin basic protein-pulsed dendritic cells
- Source :
- Journal of neuroimmunology. 172(1-2)
- Publication Year :
- 2005
-
Abstract
- We previously demonstrated that injection of myelin basic protein-pulsed (MBP-pulsed)–but not of unpulsed–autologous bone marrow-derived dendritic cells (DC) efficiently prevents experimental autoimmune encephalomyelitis (EAE) in Lewis rats. To define the molecules involved, we used 3 groups of rats pretreated subcutaneously with MBP-DC, or unpulsed DC, or PBS (control EAE). Four weeks later, all rats were immunized with encephalitogenic MBP peptide and adjuvant. Microarray analyses were done to screen for genes that differ among the 3 groups. Based on microarray analysis data, we used real-time PCR to measure expression of six probably involved genes in draining lymph node cells obtained on day 0, day 7 and day 14 post immunization (p.i.). Two of these 6 genes were consistently altered in both microarray analyses and RT-PCR. They are CD24 antigen being persistently low, and myosin light polypeptide 2 (Myl2) being high in the acute immune response in MBP-DC pretreated rats that develop resistance to EAE. These two genes could be targeted to treat EAE and, possibly, multiple sclerosis.
- Subjects :
- Encephalomyelitis, Autoimmune, Experimental
Myosin Light Chains
Time Factors
Immunology
Bone Marrow Cells
Biology
Myelin
Immune system
Antigen
Myosin
medicine
Immunology and Allergy
Animals
RNA, Messenger
Oligonucleotide Array Sequence Analysis
Microarray analysis techniques
Reverse Transcriptase Polymerase Chain Reaction
Multiple sclerosis
Experimental autoimmune encephalomyelitis
CD24 Antigen
Myelin Basic Protein
Dendritic Cells
medicine.disease
Molecular biology
Myelin basic protein
Rats
Disease Models, Animal
medicine.anatomical_structure
Neurology
Gene Expression Regulation
Rats, Inbred Lew
biology.protein
Female
Neurology (clinical)
Peptides
Cardiac Myosins
Subjects
Details
- ISSN :
- 01655728
- Volume :
- 172
- Issue :
- 1-2
- Database :
- OpenAIRE
- Journal :
- Journal of neuroimmunology
- Accession number :
- edsair.doi.dedup.....7a342022f5430f1f8db79346e434e705