Shewanella indica sp. nov., isolated from sediment of the Arabian Sea

A Gram-negative, facultatively anaerobic, rod-shaped, catalase- and oxidase-positive bacterium, motile by means of a single polar flagellum and designated strain KJW27T, was isolated from the marine sediment of Karwar jetty, west coast of India. The strain was β-haemolytic and grew with 0–10 % (w/v) NaCl, at 10–45 °C and at pH 6.5–10, with optimum growth with 2 % (w/v) NaCl, at 37 °C and at pH 7.5. The major fatty acids were iso-C15 : 0 (22.2 %), C17 : 1ω8c (21 %), summed feature 3 (comprising C16 : 1ω7c and/or C16:1ω6c; 10.2 %), C16 : 0 (7.1 %), iso-C13 : 0 (5.6 %) and C17 : 0 (4.4 %). The DNA G+C content was 51.2 mol%. Phylogenetic analysis based on 16S rRNA and gyrB gene sequences showed that strain KJW27T forms a lineage within the genus Shewanella and is closely related to Shewanella algae ATCC 51192T (98.8 %), Shewanella haliotis DW01T (98.8 %) and Shewanella chilikensis JC5T (98.2 %). Sequence identity with other members of this genus ranges from 92.2 to 96.4 %. The DNA–DNA relatedness of strain KJW27T with S. algae ATCC 51192T, S. haliotis DW01T and S. chilikensis JC5T was 52, 44 and 33 %, respectively. The phenotypic, genotypic and DNA–DNA relatedness data indicate that strain KJW27T should be distinguished from S. algae ATCC 51192T, S. haliotis DW01T and S. chilikensis JC5T. On the basis of the data presented in this study, strain KJW27T represents a novel species, for which the name Shewanella indica sp. nov. is proposed. The type strain is KJW27T ( = KCTC 23171T = BCC 41031T = NCIM 5388T).