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Measurement of peroxiredoxin-4 serum levels in rat tissue and its use as a potential marker for hepatic disease
- Source :
- Molecular Medicine Reports. 6:379-384
- Publication Year :
- 2012
- Publisher :
- Spandidos Publications, 2012.
-
Abstract
- Peroxiredoxin (Prx)-4, a secretable endoplasmic reticulum (ER)-resident isoform of the mammalian Prx family, functions as a thioredoxin-dependent peroxidase. It is acknowledged that Prx-4 plays a role in the detoxification of hydrogen peroxide, and potentially other peroxides, which may be generated during the oxidative folding of proteins and oxidative stress in the ER. The present study was undertaken in order to specifically quantify the tissue levels of Prx-4. To accomplish this, an enzyme-linked immunosorbent assay was developed using a specific polyclonal antibody produced by immunizing a rabbit with native recombinant rat Prx-4 protein. The assay was used to detect Prx-4 in the range of 0.1 and 10 ng/ml, and to investigate tissue distribution in rats. Using this immunoassay, we found that the serum levels of Prx-4 were substantially lower in asymptomatic Long-Evans Cinnamon rats, a rat model of Wilson's disease, compared to normal rats. In addition, the treatment of rat hepatoma cells with N-acetylcysteine led to a significant increase in the release of Prx-4 protein into the medium; thus, it appears likely that the secretion of Prx-4 is associated with the redox state within cells. These results suggest that serum Prx-4 has potential for use as a biomarker for hepatic oxidative stress.
- Subjects :
- Male
Cancer Research
Carcinoma, Hepatocellular
Enzyme-Linked Immunosorbent Assay
medicine.disease_cause
Biochemistry
Antibodies
Diabetes Mellitus, Experimental
Rats, Sprague-Dawley
Antibody Specificity
Cell Line, Tumor
Biomarkers, Tumor
Genetics
medicine
Animals
Secretion
Rats, Wistar
Molecular Biology
Rats, Inbred LEC
Peroxiredoxin-4
medicine.diagnostic_test
biology
Liver Diseases
Endoplasmic reticulum
Vaccination
Peroxiredoxins
Molecular biology
Recombinant Proteins
Acetylcysteine
Culture Media
Rats
Oxidative Stress
Liver
Oncology
Cell culture
Immunoglobulin G
Immunoassay
biology.protein
Molecular Medicine
Female
Rabbits
Peroxiredoxin
Oxidation-Reduction
Oxidative stress
Peroxidase
Subjects
Details
- ISSN :
- 17913004 and 17912997
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Molecular Medicine Reports
- Accession number :
- edsair.doi.dedup.....7a20dcac8673c1ce4629fc2b1d84243d