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2',3'-cyclic nucleotide 3'-phosphodiesterases inhibit hepatitis B virus replication
- Source :
- PLoS ONE, PLoS ONE, Vol 8, Iss 11, p e80769 (2013)
- Publication Year :
- 2013
-
Abstract
- 2’,3’-cyclic nucleotide 3’-phosphodiesterase (CNP) is a member of the interferon-stimulated genes, which includes isoforms CNP1 and CNP2. CNP1 is locally expressed in the myelin sheath but CNP2 is additionally expressed at low levels outside the nervous system. CNPs regulate multiple cellular functions and suppress protein production by association with polyadenylation of mRNA. Polyadenylation of Hepatitis B virus (HBV) RNAs is crucial for HBV replication. Whether CNPs interact with polyadenylation signal of HBV RNAs and interfere HBV replication is unknown. In this study, we evaluated expressions of CNP isoforms in hepatoma cell lines and their effects on HBV replication. We found that CNP2 is moderately expressed and gently responded to interferon treatment in HepG2, but not in Huh7 cells. The CNP1 and CNP2 potently inhibited HBV production by blocking viral proteins synthesis and reducing viral RNAs, respectively. In chronic hepatitis B patients, CNP was expressed in most of HBV-infected hepatocytes of liver specimens. Knockdown of CNP expression moderately improved viral production in the HepG2.2.15 cells treated with IFN-α. In conclusion, CNP might be a mediator of interferon-induced response against HBV.
- Subjects :
- Hepatitis B virus
Polyadenylation
Intracellular Space
lcsh:Medicine
Gene Expression
Biology
medicine.disease_cause
Virus Replication
Hepatitis B virus PRE beta
2',3'-Cyclic-nucleotide 3'-phosphodiesterase
Cell Line
Hepatitis B, Chronic
Interferon
2',3'-Cyclic Nucleotide 3'-Phosphodiesterase
Gene expression
medicine
Humans
lcsh:Science
Gene
Multidisciplinary
lcsh:R
Virology
Molecular biology
digestive system diseases
Isoenzymes
Protein Transport
Viral replication
Liver
Gene Knockdown Techniques
lcsh:Q
medicine.drug
Research Article
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 8
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....7a1e6304cb667f0729e728faacf6b08e