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Determination of the coumarin derivative cloricromene acid in rabbit plasma and platelets
- Source :
- Europe PubMed Central
-
Abstract
- Two methods for the determination of cloricromene acid in biological samples are described. Cloricromene acid is a catabolite of cloricromene, a coumarin derivative which is active in the cardiovascular system. After oral administration of cloricromene to a rabbit, plasma and platelets were taken at different times and cloricromene acid was then isolated by solid-phase extraction with Sep-Pak C18 cartridges using acetonitrile-tetrahydrofuran-20% aqueous acetic acid (15:11:74, v/v/v) as eluent. The analyses were performed by reversed-phase high-performance liquid chromatography (RP-HPLC) combined with fluorescence detection with excitation at 310 nm and emission at 390 nm. The limit of quantification by RP-HPLC was about 50 pg. The catabolite in the plasma was identified by continuous-flow fast atom bombardment mass spectrometry (CF-FAB-MS), also used as a complementary means of RP-HPLC determination. The results obtained by RP-HPLC and CF-FAB-MS showed good agreement.
- Subjects :
- Detection limit
Blood Platelets
Male
Aqueous solution
Chromatography
Chemistry
Chromonar
General Chemistry
Fast atom bombardment
Spectrometry, Mass, Fast Atom Bombardment
Coumarin
Mass spectrometry
High-performance liquid chromatography
Acetic acid
chemistry.chemical_compound
Column chromatography
Animals
Indicators and Reagents
Rabbits
Chromatography, High Pressure Liquid
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Europe PubMed Central
- Accession number :
- edsair.doi.dedup.....7a146938850b0b8e8aa437910d33dc94