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Transcriptome dataset of trunk neural crest cells migrating along the ventral pathway of chick embryos
- Source :
- Data in Brief, Data in Brief, Vol 21, Iss, Pp 2547-2553 (2018)
- Publication Year :
- 2018
- Publisher :
- Elsevier, 2018.
-
Abstract
- We present a transcriptome dataset generated from migratory chick trunk neural crest cells, which are destined to form components of the peripheral nervous system. Using the Sox10E1 enhancer, which specifically labels neural crest cells migrating on the trunk ventral pathway, we performed fluorescence activated cell sorting (FACS) of electroporated embryos to obtain a pure population of these cells for library preparation and Illumina sequencing. The results provide a list of genes that are enriched in the trunk neural crest. To validate the data, we performed in situ hybridization to visualize expression of selected transcripts.
- Subjects :
- 0301 basic medicine
education.field_of_study
Multidisciplinary
animal structures
Population
Neural crest
Embryo
In situ hybridization
Biology
lcsh:Computer applications to medicine. Medical informatics
Trunk
Cell biology
Transcriptome
03 medical and health sciences
030104 developmental biology
Biochemistry, Genetics and Molecular Biology
embryonic structures
lcsh:R858-859.7
education
Enhancer
lcsh:Science (General)
Illumina dye sequencing
lcsh:Q1-390
Subjects
Details
- Language :
- English
- ISSN :
- 23523409
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- Data in Brief
- Accession number :
- edsair.doi.dedup.....79f891ed2aa5c363056acf9090d38d32