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Transcriptome dataset of trunk neural crest cells migrating along the ventral pathway of chick embryos

Authors :
Felipe Monteleone Vieceli
Christina Murko
Marianne E. Bronner
Source :
Data in Brief, Data in Brief, Vol 21, Iss, Pp 2547-2553 (2018)
Publication Year :
2018
Publisher :
Elsevier, 2018.

Abstract

We present a transcriptome dataset generated from migratory chick trunk neural crest cells, which are destined to form components of the peripheral nervous system. Using the Sox10E1 enhancer, which specifically labels neural crest cells migrating on the trunk ventral pathway, we performed fluorescence activated cell sorting (FACS) of electroporated embryos to obtain a pure population of these cells for library preparation and Illumina sequencing. The results provide a list of genes that are enriched in the trunk neural crest. To validate the data, we performed in situ hybridization to visualize expression of selected transcripts.

Details

Language :
English
ISSN :
23523409
Volume :
21
Database :
OpenAIRE
Journal :
Data in Brief
Accession number :
edsair.doi.dedup.....79f891ed2aa5c363056acf9090d38d32