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Symbiotic polyamine metabolism regulates epithelial proliferation and macrophage differentiation in the colon
- Source :
- Nature Communications, Vol 12, Iss 1, Pp 1-14 (2021), Nature Communications
- Publication Year :
- 2021
- Publisher :
- Nature Portfolio, 2021.
-
Abstract
- Intestinal microbiota-derived metabolites have biological importance for the host. Polyamines, such as putrescine and spermidine, are produced by the intestinal microbiota and regulate multiple biological processes. Increased colonic luminal polyamines promote longevity in mice. However, no direct evidence has shown that microbial polyamines are incorporated into host cells to regulate cellular responses. Here, we show that microbial polyamines reinforce colonic epithelial proliferation and regulate macrophage differentiation. Colonisation by wild-type, but not polyamine biosynthesis-deficient, Escherichia coli in germ-free mice raises intracellular polyamine levels in colonocytes, accelerating epithelial renewal. Commensal bacterium-derived putrescine increases the abundance of anti-inflammatory macrophages in the colon. The bacterial polyamines ameliorate symptoms of dextran sulfate sodium-induced colitis in mice. These effects mainly result from enhanced hypusination of eukaryotic initiation translation factor. We conclude that bacterial putrescine functions as a substrate for symbiotic metabolism and is further absorbed and metabolised by the host, thus helping maintain mucosal homoeostasis in the intestine.<br />Polyamines produced by intestinal bacteria are thought to have beneficial effects on the host. Here the authors show that these polyamines increase regulatory macrophage abundance and are taken up by colonic epithelial cells to enhance colonic barrier function and immunity in mice.
- Subjects :
- 0301 basic medicine
Male
Colon
Science
General Physics and Astronomy
General Biochemistry, Genetics and Molecular Biology
Article
03 medical and health sciences
chemistry.chemical_compound
Mice
0302 clinical medicine
Peptide Initiation Factors
medicine
Escherichia coli
Putrescine
Animals
Homeostasis
Translation factor
Colitis
Intestinal Mucosa
Symbiosis
Cell Proliferation
Multidisciplinary
Chemistry
Macrophages
Dextran Sulfate
RNA-Binding Proteins
Cell Differentiation
Epithelial Cells
General Chemistry
Metabolism
medicine.disease
digestive system diseases
Cell biology
Gastrointestinal Microbiome
Spermidine
Mice, Inbred C57BL
030104 developmental biology
030220 oncology & carcinogenesis
Mucosal immunology
Female
Microbiome
Polyamine
Intracellular
Subjects
Details
- Language :
- English
- ISSN :
- 20411723
- Volume :
- 12
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Nature Communications
- Accession number :
- edsair.doi.dedup.....79e77ede725cbc3b5e79582c55386c44