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Online SERS detection and characterization of eight biologically-active peptides separated by capillary zone electrophoresis
- Source :
- The Analyst. 140:1516-1522
- Publication Year :
- 2015
- Publisher :
- Royal Society of Chemistry (RSC), 2015.
-
Abstract
- There is a need for low cost, sensitive and chemical specific detectors for routine characterization of biomolecules. In this study, we utilize sheath-flow surface-enhanced Raman scattering (SERS) to analyze a mixture of eight biologically-active peptides separated by capillary zone electrophoresis (CZE). Analysis of the SERS electropherogram resulting from online detection resolves the characteristic Raman bands attributed to the amino acid constituents of each peptide, which enables identification. The detection limit by SERS was found to be 10−8 M. Our results suggest that the structural information obtained from the detected vibrational modes provides complementary characterization to other chemically specific detectors like mass spectrometry and improved chemical identification over other commonly used optical-based post-chromatographic detection methods. In addition, the sheath-flow SERS detection results in band narrowing in the observed electropherogram that enables distinction of closely migrating species. The results presented here indicate that online SERS detection can provide fast, robust, reproducible, and chemical specific detection to facilitate the characterization of peptides.
- Subjects :
- Spectrometry, Mass, Electrospray Ionization
Analytical chemistry
Peptide
Spectrum Analysis, Raman
010402 general chemistry
Mass spectrometry
Online Systems
01 natural sciences
Biochemistry
Article
Analytical Chemistry
symbols.namesake
Capillary electrophoresis
Electrochemistry
Humans
Environmental Chemistry
Spectroscopy
chemistry.chemical_classification
Detection limit
Chromatography
Chemistry
Biomolecule
010401 analytical chemistry
Electrophoresis, Capillary
Peptide Fragments
0104 chemical sciences
Characterization (materials science)
Electropherogram
symbols
Raman scattering
Subjects
Details
- ISSN :
- 13645528 and 00032654
- Volume :
- 140
- Database :
- OpenAIRE
- Journal :
- The Analyst
- Accession number :
- edsair.doi.dedup.....79de5432816a42a7f4b8fe9394feff0e