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In vivo functional dissection of human inner kinetochore protein CENP-C
- Source :
- Journal of Structural Biology. 140:39-48
- Publication Year :
- 2002
- Publisher :
- Elsevier BV, 2002.
-
Abstract
- CENP-C is a fundamental component of the inner kinetochore plate and contributes to the formation of functional centromeres in eukaryotic organisms. Recruitment of CENP-C to kinetochore requires other centromere proteins, particularly CENP-A, CENP-H, and CENP-I. However, how CENP-C is correctly localized at the kinetochore is not clearly determined, mainly due to the functional variety of its domains, which hints at a complex recruitment mechanism. Here, by both immunofluorescent labeling and chromatin/immunoprecipitation we could show that human CENP-C contains two distinct domains, one in the central region, between amino acids 426 and 537, and the second one in the carboxyl terminal region, between amino acids 638 and 943, which are both capable of localizing at centromeres and binding α-satellite DNA. The presence of two domains that iterate the same function despite being significantly different in their amino acid sequence and structure suggests that CENP-C may target the centromere by establishing multiple contacts with both the DNA and protein constituents of the kinetochore. © 2002 Elsevier Science (USA). All rights reserved.
- Subjects :
- Chromosomal Proteins, Non-Histone
Immunoprecipitation
Blotting, Western
Centromere
macromolecular substances
DNA, Satellite
Biology
α-Satellite DNA
DNA binding domain
Protein structure
Structural Biology
Formaldehyde
Humans
Kinetochores
Peptide sequence
Centromere localization domain
Kinetochore
DNA-binding domain
Precipitin Tests
Chromatin
Protein Structure, Tertiary
Cell biology
Cross-Linking Reagents
Microscopy, Fluorescence
Structural biology
Biochemistry
Mutation
Electrophoresis, Polyacrylamide Gel
CENP-C
Subjects
Details
- ISSN :
- 10478477
- Volume :
- 140
- Database :
- OpenAIRE
- Journal :
- Journal of Structural Biology
- Accession number :
- edsair.doi.dedup.....79c4da75acf482777f439ac40804e39c