Transformation of Bovine Cells in vitro by Polyoma Virus, and the Properties of the Transformed Cells

SummaryCell cultures of bovine embryonic lung were found to undergo morphological changes after inoculation with polyoma virus. The changes were characteristic for polyoma transformation with cells having stellate or triangular shape and lying at random, crisscrossing one another.Cell lines of rapidly-growing transformed cells were obtained. Attempts to isolate infectious virus from the lines were negative. The lines contained “tumor” antigen as demonstrated by complement fixation tests using serum from hamsters bearing polyoma-in-duced tumors.A comparison of the viral susceptibility between the polyoma-trans formed cells and SV40-transformed and normal cells derived from the same source revealed certain differences. Whereas the polyoma-transformed cells showed fairly high susceptibility to a wild strain of type C of foot-and-mouth disease virus (FMDV) the SV40-transformed and normal cells were less susceptible. The SV40-transformed cells showed a decreased susceptibility to bovine enterovirus (BEV) and p...