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Ins1 Cre knock-in mice for beta cell-specific gene recombination
- Source :
- Diabetologia, Diabetologia, vol. 58, no. 3, pp. 558-565
- Publication Year :
- 2013
-
Abstract
- Aims/hypothesis Pancreatic beta cells play a central role in the control of glucose homeostasis by secreting insulin to stimulate glucose uptake by peripheral tissues. Understanding the molecular mechanisms that control beta cell function and plasticity has critical implications for the pathophysiology and therapy of major forms of diabetes. Selective gene inactivation in pancreatic beta cells, using the Cre-lox system, is a powerful approach to assess the role of particular genes in beta cells and their impact on whole body glucose homeostasis. Several Cre recombinase (Cre) deleter mice have been established to allow inactivation of genes in beta cells, but many show non-specific recombination in other cell types, often in the brain. Methods We describe the generation of Ins1Cre and Ins1CreERT2 mice in which the Cre or Cre-oestrogen receptor fusion protein (CreERT2) recombinases have been introduced at the initiation codon of the Ins1 gene. Results We show that Ins1Cre mice induce efficient and selective recombination of floxed genes in beta cells from the time of birth, with no recombination in the central nervous system. These mice have normal body weight and glucose homeostasis. Furthermore, we show that tamoxifen treatment of adult Ins1CreERT2 mice crossed with Rosa26-tdTomato mice induces efficient recombination in beta cells. Conclusions/interpretation These two strains of deleter mice are useful new resources to investigate the molecular physiology of pancreatic beta cells. Electronic supplementary material The online version of this article (doi:10.1007/s00125-014-3468-5) contains peer-reviewed but unedited supplementary material, which is available to authorised users.
- Subjects :
- Male
Cell type
medicine.medical_specialty
Endocrinology, Diabetes and Metabolism
Glucose uptake
Pancreatic islets
Hypothalamus
Cre recombinase
Beta cells
Glucose homeostasis
Insulin
Transgenic mice
030209 endocrinology & metabolism
Biology
Article
03 medical and health sciences
Mice
0302 clinical medicine
Internal medicine
Gene knockin
Insulin-Secreting Cells
Internal Medicine
medicine
Animals
030304 developmental biology
0303 health sciences
Glucose tolerance test
medicine.diagnostic_test
Integrases
Glucose Tolerance Test
Mice, Mutant Strains
3. Good health
Cell biology
Endocrinology
medicine.anatomical_structure
Female
Beta cell
Subjects
Details
- Volume :
- 58
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Diabetologia
- Accession number :
- edsair.doi.dedup.....79acc7438145f6d5e8e41807bed52ee3
- Full Text :
- https://doi.org/10.1007/s00125-014-3468-5