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SiFBA5, a cold-responsive factor from Saussurea involucrata promotes cold resilience and biomass increase in transgenic tomato plants under cold stress
- Source :
- BMC Plant Biology, Vol 21, Iss 1, Pp 1-10 (2021), BMC Plant Biology
- Publication Year :
- 2021
- Publisher :
- BMC, 2021.
-
Abstract
- Background Saussurea involucrata survives in extreme arctic conditions and is very cold-resistant. This species grows in rocky, mountainous areas with elevations of 2400–4100 m, which are snow-covered year-round and are subject to freezing temperatures. S. involucrata’s ability to survive in an extreme low-temperature environment suggests that it has particularly high photosynthetic efficiency, providing a magnificent model, and rich gene pool, for the analysis of plant cold stress response. Fructose-1, 6-bisphosphate aldolase (FBA) is a key enzyme in the photosynthesis process and also mediates the conversion of fructose 1, 6-bisphosphate (FBP) into dihydroxyacetone phosphate (DHAP) and glycerol triphosphate (GAP) during glycolysis and gluconeogenesis. The molecular mechanisms underlying S. involucrata’s cold tolerance are still unclear; therefore, our work aims to investigate the role of FBA in plant cold-stress response. Results In this study, we identified a cold-responsive gene, SiFBA5, based on a preliminary low-temperature, genome-wide transcriptional profiling of S. involucrata. Expression analysis indicated that cold temperatures rapidly induced transcriptional expression of SiFBA5, suggesting that SiFBA5 participates in the initial stress response. Subcellular localization analysis revealed that SiFBA5 is localized to the chloroplast. Transgenic tomato plants that overexpressed SiFBA5 were generated using a CaMV 35S promoter. Phenotypic observation suggested that the transgenic plants displayed increased cold tolerance and photosynthetic efficiency in comparison with wild-type plants. Conclusion Cold stress has a detrimental impact on crop yield. Our results demonstrated that SiFBA5 positively regulates plant response to cold stress, which is of great significance for increasing crop yield under cold stress conditions.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Saussurea
Chlorophyll
Transcription, Genetic
Plant Science
Genetically modified crops
Photosynthetic efficiency
Biology
Photosynthesis
01 natural sciences
Fluorescence
03 medical and health sciences
chemistry.chemical_compound
Solanum lycopersicum
Gene Expression Regulation, Plant
Stress, Physiological
lcsh:Botany
Botany
Genetically modified tomato
Amino Acid Sequence
Biomass
Phylogeny
Dihydroxyacetone phosphate
Plant Proteins
Aldolase A
food and beverages
Plants, Genetically Modified
SiFBA5
lcsh:QK1-989
Chloroplast
Cold Temperature
030104 developmental biology
Calvin cycle
chemistry
biology.protein
Cold stress
010606 plant biology & botany
Research Article
Subcellular Fractions
Subjects
Details
- Language :
- English
- ISSN :
- 14712229
- Volume :
- 21
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- BMC Plant Biology
- Accession number :
- edsair.doi.dedup.....79a3e1154577f0ced09439ee1bb6d790