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Persistence of Staphylococcus aureus L-form during experimental lung infection in rats
- Source :
- FEMS microbiology letters. 268(1)
- Publication Year :
- 2006
-
Abstract
- The course of pulmonary infection in rats infected by intranasal inoculation with a Staphylococcus aureus stable protoplast L-form was studied. Blood and bronchoalveolar samples were taken on days 3, 7, 14 and 30 after challenge and were investigated by microbiological, electron-microscopic, cytochemical and cytometric methods. The electron microscopic data and isolation of L-form cultures from bronchoalveolar samples at all experimental times demonstrated the ability of S. aureus L-form cells to internalize, replicate and persist in the lungs of infected rats to the end of the observation period, in contrast to the S. aureus parental form. It was found that persisting L-form evoked ineffectual phagocytose by alveolar macrophages and low but long-lasting inflammatory reaction in rats. The experimental model of pulmonary infection with S. aureus L-form suggests that the cell-wall-deficient bacterial forms may be involved in the pathogenesis of chronic and latent lung infections.
- Subjects :
- Male
Microscopy, Electron, Scanning Transmission
Staphylococcus aureus
Phagocytosis
L Forms
Biology
medicine.disease_cause
Microbiology
Pathogenesis
L-form bacteria
Microscopy, Electron, Transmission
Macrophages, Alveolar
Pneumonia, Staphylococcal
Genetics
medicine
Animals
Rats, Wistar
Molecular Biology
Lung
Inoculation
Protoplast
Rats
medicine.anatomical_structure
Immunology
Nasal administration
Female
Bronchoalveolar Lavage Fluid
Subjects
Details
- ISSN :
- 03781097
- Volume :
- 268
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- FEMS microbiology letters
- Accession number :
- edsair.doi.dedup.....79a2bc26f587879855775cec45c2d4f7