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Construction of integrated gene logic-chip
- Source :
- Nature nanotechnology. 13(10)
- Publication Year :
- 2017
-
Abstract
- In synthetic biology, the control of gene expression requires a multistep processing of biological signals. The key steps are sensing the environment, computing information and outputting products1. To achieve such functions, the laborious, combinational networking of enzymes and substrate-genes is required, and to resolve problems, sophisticated design automation tools have been introduced2. However, the complexity of genetic circuits remains low because it is difficult to completely avoid crosstalk between the circuits. Here, we have made an orthogonal self-contained device by integrating an actuator and sensors onto a DNA origami-based nanochip that contains an enzyme, T7 RNA polymerase (RNAP) and multiple target-gene substrates. This gene nanochip orthogonally transcribes its own genes, and the nano-layout ability of DNA origami allows us to rationally design gene expression levels by controlling the intermolecular distances between the enzyme and the target genes. We further integrated reprogrammable logic gates so that the nanochip responds to water-in-oil droplets and computes their small RNA (miRNA) profiles, which demonstrates that the nanochip can function as a gene logic-chip. Our approach to component integration on a nanochip may provide a basis for large-scale, integrated genetic circuits.
- Subjects :
- 0301 basic medicine
Models, Molecular
Small RNA
Transcription, Genetic
Computer science
Biomedical Engineering
Gene Expression
Bioengineering
Computational biology
03 medical and health sciences
Synthetic biology
Computers, Molecular
Viral Proteins
medicine
DNA origami
T7 RNA polymerase
General Materials Science
Electrical and Electronic Engineering
Gene
Oligonucleotide Array Sequence Analysis
DNA
DNA-Directed RNA Polymerases
Condensed Matter Physics
Chip
Atomic and Molecular Physics, and Optics
Nanostructures
MicroRNAs
030104 developmental biology
Logic gate
Electronic design automation
Synthetic Biology
medicine.drug
Subjects
Details
- ISSN :
- 17483395
- Volume :
- 13
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Nature nanotechnology
- Accession number :
- edsair.doi.dedup.....7999a44657a4fd8df03fb55e188c903d