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Staphylococcus aureus antigens reactive with milk immunoglobulin G of naturally infected dairy cows
- Source :
- Journal of Clinical Microbiology. 26:1175-1180
- Publication Year :
- 1988
- Publisher :
- American Society for Microbiology, 1988.
-
Abstract
- A 14- to 26-kilodalton fraction of Staphylococcus aureus exoproteins isolated by molecular sieve chromatography and electroelution from polyacrylamide gels was shown to specifically react with antibodies in milk of naturally infected dairy cows. Silver staining of the antigen preparation electrophoresed in polyacrylamide gels showed the strongest reactivity in the 24- to 26-kilodalton region with lesser staining at lower apparent molecular sizes. An enzyme-linked immunosorbent assay was developed to differentiate infected from uninfected cows for diagnostic purposes. Samples from S. aureus-infected cows reacted in the assay, and samples from uninfected cows did not. There was no correlation between numbers of somatic cells in the samples and reactivity to the antigens. Samples from cows infected with coagulase-negative staphylococci did not react with the antigens. It was found, however, that some samples from uninfected cows that were recently postpartum or producing low amounts of milk contained antibodies which bound the antigens. This was believed to be due to transport from blood to the mammary gland of antibodies which were generated by previous intramammary infections or infections at other sites.
- Subjects :
- Microbiology (medical)
Staphylococcus aureus
Cattle Diseases
Biology
Staphylococcal infections
medicine.disease_cause
Immunoglobulin G
Microbiology
Silver stain
Antigen
medicine
Animals
False Positive Reactions
Antigens, Bacterial
food and beverages
Staphylococcal Infections
Milk Proteins
medicine.disease
Staining
Molecular Weight
Electroelution
biology.protein
Cattle
Electrophoresis, Polyacrylamide Gel
Female
Antibody
Research Article
Subjects
Details
- ISSN :
- 1098660X and 00951137
- Volume :
- 26
- Database :
- OpenAIRE
- Journal :
- Journal of Clinical Microbiology
- Accession number :
- edsair.doi.dedup.....7995d1872d0ae7c9a1e0274eea81e427