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Prevalence and molecular characterization of methicillin-resistant Staphylococcus aureus with mupirocin, fusidic acid and/or retapamulin resistance
- Source :
- BMC Microbiology, Vol 20, Iss 1, Pp 1-12 (2020), BMC Microbiology
- Publication Year :
- 2020
- Publisher :
- Research Square Platform LLC, 2020.
-
Abstract
- Background The data on the prevalence of resistance to mupirocin (MUP), fusidic acid (FA) and retapamulin (RET) in methicillin-resistant Staphylococcus aureus (MRSA) from China are still limited. This study aimed to examine these three antibiotics resistance in 1206 MRSA clinical isolates from Eastern China. Phenotypic MUP, FA and RET resistance was determined by minimum inhibitory concentrations (MICs), and genotypic by PCR and DNA sequencing of the mupA/B, fusB-D, cfr, vgaA/Av/ALC/B/C/E, lsaA-C/E and salA and mutations in ileS, fusA/E, rplC, and 23S RNA V domain. The genetic characteristics of resistance isolates were conducted by pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Results Overall MRSA MUP, FA and RET resistance was low (5.1, 1.0 and 0.3%, respectively). MupA was the mechanism of high-level MUP resistance. All low-level MUP resistance isolates possessed an equivocal mutation N213D in IleS; of these, 2 reported an additional V588F mutation with an impact on the Rossman fold. FusA mutations, such as L461K, H457Q, H457Y and V90I were the primary FA mechanisms among high-level resistance isolates, most of which also contained fusC; however, all low-level resistance strains carried fusB. Except lsaE gene detected in one isolate, no other resistance mechanisms tested were found among RET-resistant isolates. Additionally, sixteen PFGE types (A-P) were observed, among which type B was the most common (49/76, 64.5%), followed by types E and G (4/76, 5.3% each) and types C and M (3/76, 3.9% each). All resistant strains were divided into 15 ST types by MLST. ST764 (24/76, 31.6%), ST630 (11/76, 14.5%), ST239 (9/76, 11.8%) and ST5 (7/76, 9.2%) were the major types. PFGE type B isolates with the aforementioned STs were mainly found in mupirocin resistant isolates. Conclusions MUP, FA and RET exhibited highly activity against the MRSA isolates. Acquired genes and chromosome-borne genes mutations were responsible for MUP and FA resistance; however, the mechanism for some RET-resistant isolates remains to be further elucidated. Also, the surveillance to MUP in MRSA should be strengthened to prevent elevated resistance due to the expansion of clones.
- Subjects :
- Microbiology (medical)
Retapamulin
DNA, Bacterial
China
Fusidic acid
lcsh:QR1-502
Mupirocin
Microbial Sensitivity Tests
Biology
medicine.disease_cause
Resistance mechanisms
Microbiology
lcsh:Microbiology
03 medical and health sciences
chemistry.chemical_compound
Antibiotic resistance
Drug Resistance, Multiple, Bacterial
Genotype
medicine
Pulsed-field gel electrophoresis
Prevalence
Humans
030304 developmental biology
0303 health sciences
030306 microbiology
Genetic characteristics
Sequence Analysis, DNA
Staphylococcal Infections
Bridged Bicyclo Compounds, Heterocyclic
Methicillin-resistant Staphylococcus aureus
Anti-Bacterial Agents
Bacterial Typing Techniques
Electrophoresis, Gel, Pulsed-Field
chemistry
Mutation
Multilocus sequence typing
Diterpenes
medicine.drug
Research Article
Multilocus Sequence Typing
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- BMC Microbiology, Vol 20, Iss 1, Pp 1-12 (2020), BMC Microbiology
- Accession number :
- edsair.doi.dedup.....798cd5cbe46ebfb2cdb162b839e83606