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Innate and introduced resistance traits in genetically modified aspen trees and their effect on leaf beetle feeding
- Source :
- PLoS ONE, PLoS ONE, Public Library of Science, 2013, 8 (9), 8 p. ⟨10.1371/journal.pone.0073819⟩, PLoS ONE, Vol 8, Iss 9, p e73819 (2013), Plos One 9 (8), 8 p.. (2013)
- Publication Year :
- 2013
- Publisher :
- HAL CCSD, 2013.
-
Abstract
- Genetic modifications of trees may provide many benefits, e. g. increase production, and mitigate climate change and herbivore impacts on forests. However, genetic modifications sometimes result in unintended effects on innate traits involved in plant-herbivore interactions. The importance of intentional changes in plant defence relative to unintentional changes and the natural variation among clones used in forestry has not been evaluated. By a combination of biochemical measurements and bioassays we investigated if insect feeding on GM aspens is more affected by intentional (induction Bt toxins) than of unintentional, non-target changes or clonal differences in innate plant defence. We used two hybrid wildtype clones (Populus tremula x P. tremuloides and Populus tremula x P. alba) of aspen that have been genetically modified for 1) insect resistance (two Bt lines) or 2) reduced lignin properties (two lines COMT and CAD), respectively. Our measurements of biochemical properties suggest that unintended changes by GM modifications (occurring due to events in the transformation process) in innate plant defence (phenolic compounds) were generally smaller but fundamentally different than differences seen among different wildtype clones (e. g. quantitative and qualitative, respectively). However, neither clonal differences between the two wildtype clones nor unintended changes in phytochemistry influenced consumption by the leaf beetle (Phratora vitellinae). By contrast, Bt induction had a strong direct intended effect as well as a post experiment effect on leaf beetle consumption. The latter suggested lasting reduction of beetle fitness following Bt exposure that is likely due to intestinal damage suffered by the initial Bt exposure. We conclude that Bt induction clearly have intended effects on a target species. Furthermore, the effect of unintended changes in innate plant defence traits, when they occur, are context dependent and have in comparison to Bt induction probably less pronounced effect on targeted herbivores.
- Subjects :
- 0106 biological sciences
Phratora vitellinae
Genetics and Breeding
résistance aux insectes
Science
[SDV]Life Sciences [q-bio]
Context (language use)
Genetically modified crops
Plant disease resistance
010603 evolutionary biology
01 natural sciences
Host-Parasite Interactions
Phenols
chrysomela tremulae
Botany
Animals
Biologiska vetenskaper
Glycosides
Chromatography, High Pressure Liquid
Disease Resistance
Plant Diseases
Hybrid
Herbivore
Multidisciplinary
tremble
biology
fungi
Geovetenskap och miljövetenskap
Feeding Behavior
Biological Sciences
15. Life on land
Plants, Genetically Modified
biology.organism_classification
Genetically modified organism
Coleoptera
Plant Leaves
Genetik och förädling
Populus
plante transgénique
Hybridization, Genetic
Medicine
Quercetin
Earth and Related Environmental Sciences
Research Article
010606 plant biology & botany
Leaf beetle
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Database :
- OpenAIRE
- Journal :
- PLoS ONE, PLoS ONE, Public Library of Science, 2013, 8 (9), 8 p. ⟨10.1371/journal.pone.0073819⟩, PLoS ONE, Vol 8, Iss 9, p e73819 (2013), Plos One 9 (8), 8 p.. (2013)
- Accession number :
- edsair.doi.dedup.....7971e418fe7a8f4ea72c710dadcee76d