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Human Papilloma Virus (HPV)-E6/E7 and Epidermal Growth Factor Receptor (EGF-R) Protein Levels in Cervical Cancer and Cervical Intraepithelial Neoplasia (CIN)
- Source :
- American Journal of Reproductive Immunology. 46:280-287
- Publication Year :
- 2001
- Publisher :
- Wiley, 2001.
-
Abstract
- BACKGROUND: About 90% of cervical cancers and advanced cervical intraepithelial neoplasia (CIN II/III) are squamous epithelial cells with mRNA for human papillomavirus (HPV)16 and 18 and up-regulated epidermal growth factor receptor (EGF-R). Since presence of proteins rather than mRNA may be truly indicative of active infection or disease progression, establishing reliable methods for quantifying these proteins in cervical biopsies is important. METHOD: We have established an objective semi-quantitative immunofluorescent antibody assay to reliably assess the levels of HPV-E6/E7 and EGF-R proteins in the cervical biopsies from 12 normal women, five women with CIN I, 15 with CIN II/III and ten with cervical cancer. RESULTS: HPV-E6/E7 and EGF-R, when present, were specific to para-basal, basal and squamous epithelial cells (negative in stromal cells). Nine of ten women with cervical cancer and 15 (14 CIN II/III; 1 CIN I) of 20 women with CIN were positive for HPV-E6/E7. All 12 controls were HPV-negative. The controls and six women with CIN (four with CIN I) negative for HPV had low levels of EGF-R. The only exception was one woman with cervical cancer negative for HPV, with high levels of EGF-R. Levels of HPV-E6/E7 and EGF-R were significantly higher (P
- Subjects :
- Pathology
medicine.medical_specialty
Stromal cell
Papillomavirus E7 Proteins
Immunology
Uterine Cervical Neoplasms
Cervix Uteri
Cervical intraepithelial neoplasia
Basal (phylogenetics)
Epidermal growth factor
medicine
Humans
Immunology and Allergy
Epidermal growth factor receptor
Papillomaviridae
Fluorescent Antibody Technique, Indirect
Cervical cancer
biology
virus diseases
Obstetrics and Gynecology
Epithelial Cells
Oncogene Proteins, Viral
Uterine Cervical Dysplasia
medicine.disease
biology.organism_classification
female genital diseases and pregnancy complications
DNA-Binding Proteins
ErbB Receptors
Repressor Proteins
Reproductive Medicine
Carcinoma, Squamous Cell
biology.protein
Cancer research
Female
Antibody
Subjects
Details
- ISSN :
- 16000897 and 87558920
- Volume :
- 46
- Database :
- OpenAIRE
- Journal :
- American Journal of Reproductive Immunology
- Accession number :
- edsair.doi.dedup.....796848a9ed8ac15af3bb01ce45df8b3d