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Enzyme Immunoassays for Measurement of Cytomegalovirus Immunoglobulin M Antibody
- Source :
- Journal of Clinical Microbiology. 14:427-432
- Publication Year :
- 1981
- Publisher :
- American Society for Microbiology, 1981.
-
Abstract
- The diagnosis of congenital cytomegalovirus (CMV) infection is often accomplished by the detection of circulating antibody directed against CMV. We devised a method for measuring CMV-specific immunoglobulin M (IgM) based on the isolation of IgM antibody by reaction with a solid phase coated with antihuman IgM. The determination of IgM antibody specific for CMV was accomplished by the subsequent addition of CMV or control antigen and enzyme-labeled CMV antibody (solid phase-IgM method). We compared the sensitivity and specificity of this method with those of a conventional form of solid-phase enzyme immunoassay in which CMV antigen is bound to the solid phase (solid phase-antigen method). Both assay systems were capable of detecting CMV-specific IgM antibody in the sera of 10 babies with documented CMV infection and in those of the mothers of 4 of these babies. The solid phase-IgM method yielded negative results in all 66 sera available from babies who did not have congenital CMV infection. On the other hand, the solid phase-antigen system yielded false-positive results in 12 (18%) of these sera. In addition, the solid phase-antigen system yielded false-positive results in 8 of 12 sera obtained from patients with demonstrable rheumatoid factor. However, the solid phase-IgM system yielded negative results for the rheumatoid sera, provided that appropriate control reactions were performed. The solid phase-IgM system is thus a specific and sensitive method for the determination of CMV IgM antibody.
- Subjects :
- Microbiology (medical)
Congenital cytomegalovirus infection
Cytomegalovirus
Antibodies, Viral
Infant, Newborn, Diseases
Immunoenzyme Techniques
Antigen
Rheumatoid Factor
medicine
Humans
Rheumatoid factor
False Positive Reactions
biology
medicine.diagnostic_test
Infant, Newborn
Infant
virus diseases
medicine.disease
Virology
Immunoglobulin M
Immunoassay
Cytomegalovirus Infections
Immunology
biology.protein
Virology, Rickettsiology, and Chlamydiology
Cytomegalovirus immunoglobulin M antibody
Enzyme immunoassays
Antibody
Subjects
Details
- ISSN :
- 1098660X and 00951137
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- Journal of Clinical Microbiology
- Accession number :
- edsair.doi.dedup.....7967f29668706b58bd1e45adfb4efe2c