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Establishment of human induced pluripotent stem cell line MUi028-A from normal fetal skin fibroblasts

Authors :
Sukanya Chumchuen
Chonthicha Satirapod
Amornrat Tangprasittipap
Suradej Hongeng
Source :
Stem Cell Research, Vol 60, Iss, Pp 102675-(2022)
Publication Year :
2021

Abstract

MUi028-A human induced pluripotent stem cell (hiPSC) line was generated from normal fetal skin fibroblasts using a non-integrative reprogramming method. Reprogramming factors OCT4, SOX2, KLF4, L-MYC, and LIN28, and TP53 shRNA in three episomal vectors were delivered by electroporation. The MUi028-A cell line had embryonic stem cell characteristics with consistent expression of specific pluripotency markers and the capability of in vitro differentiation into the three germ layers. This iPSC line can be used as a control to study disease mechanisms.

Details

ISSN :
18767753
Volume :
60
Database :
OpenAIRE
Journal :
Stem cell research
Accession number :
edsair.doi.dedup.....7967d41f8af07b3a56d2575bf915f928