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Anti-Pancreatic Cancer Effects of a Polar Extract From the Edible Sea Cucumber, Cucumaria frondosa
- Source :
- Pancreas. 39:646-652
- Publication Year :
- 2010
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2010.
-
Abstract
- Objectives To investigate the effects and mechanism of Frondanol-A5P, a polar extract from Cucumaria frondosa, on growth inhibition and apoptosis in S2013 and AsPC-1 human pancreatic cancer cells. Methods The effects of Frondanol-A5P on proliferation, cell cycle, expression of cell cycle proteins and p21, phosphorylation of MAP kinases, annexin V binding, and caspase-3 activation were examined. Results Frondanol-A5P inhibited proliferation and induced G2/M phase cell cycle arrest in both cell lines with decreased expression of cyclin A, cyclin B, and cdc25c. Frondanol-A5P induced phosphorylation of stress-activated protein kinase and Janus kinase (SAPK/JAK) and p38 mitogen-activated protein kinase (MAP) within 5 minutes. Frondanol-A5P markedly increased expression of p21 messenger RNA and protein at 3 hours in both cell lines. This effect was reduced by the p38 kinase inhibitor, SB203580. Frondanol-A5P markedly increased annexin V binding and activated caspase-3. Conclusions Frondanol-A5 causes cell cycle arrest and apoptosis in human pancreatic cancer cells. These changes are associated with decreased expression of cyclin A, cyclin B, and cdc25c and increased expression of p21 that, at least in part, is mediated by a p38 kinase-dependent mechanism. Because Frondanol-A5P is derived from an edible, nontoxic, sea cucumber, it may be valuable for nutritional therapy or prevention of pancreatic cancer.
- Subjects :
- Cell cycle checkpoint
Annexins
Endocrinology, Diabetes and Metabolism
Cyclin A
Cyclin B
Antineoplastic Agents
Apoptosis
Cell Cycle Proteins
Complex Mixtures
p38 Mitogen-Activated Protein Kinases
Endocrinology
Annexin
Cell Line, Tumor
Internal Medicine
Animals
Humans
Phosphorylation
Protein kinase A
Cell Proliferation
Hepatology
biology
Caspase 3
Chemistry
Kinase
Cell Cycle
Cucumaria
Cell cycle
Molecular biology
Pancreatic Neoplasms
biology.protein
Janus kinase
Subjects
Details
- ISSN :
- 08853177
- Volume :
- 39
- Database :
- OpenAIRE
- Journal :
- Pancreas
- Accession number :
- edsair.doi.dedup.....794c77a2fc30494ab13a68ec2f075c72