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MAG versus PEG: Incorporating a Poly(MAG) Layer to Promote Colloidal Stability of Nucleic Acid/'Click Cluster' Complexes
- Source :
- ACS macro letters. 1(5)
- Publication Year :
- 2022
-
Abstract
- Herein, we demonstrate the reversible addition–fragmentation chain transfer (RAFT) synthesis of an adamantane-conjugated glycopolymer, poly(2-methacrylamido-2-deoxy glucopyranose) (Ad-pMAG), as a hydrophilic coating to promote colloidal stability of click cluster-pDNA complexes in biological media. The Ad-pMAG is assembled via noncovalent interactions through inclusion complex formation between adamantane (Ad) and the β-cyclodextrin (βCD) core of the click cluster/pDNA and then further assembled with plasmid DNA to form polyplexes. Ad-pMAG incorporation was favorable over Ad-poly(ethylene glycol) (Ad-PEG) due to the enhanced colloidal stability of the click cluster/pDNA polyplex under physiological salt conditions at high N/P ratios. Interestingly, the uptake and reporter gene expression with polyplexes coated with the Ad-pMAG was much lower in HeLa cells than that observed with two glioma cell lines (U87 and U251 cells) in vitro, possibly indicating some delivery specificity.
- Subjects :
- chemistry.chemical_classification
Materials science
Polymers and Plastics
Stereochemistry
Glycopolymer
Adamantane
Organic Chemistry
Chain transfer
Raft
Inorganic Chemistry
chemistry.chemical_compound
chemistry
PEG ratio
Materials Chemistry
Nucleic acid
Biophysics
Non-covalent interactions
Ethylene glycol
Subjects
Details
- ISSN :
- 21611653
- Volume :
- 1
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- ACS macro letters
- Accession number :
- edsair.doi.dedup.....79406b1a63ec1c1eca341ce79ef4d55a