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Effect of global transcriptional regulators on kinetic behavior of Escherichia coli under anaerobic fermentation conditions
- Source :
- Archives of Microbiology. 200:979-987
- Publication Year :
- 2018
- Publisher :
- Springer Science and Business Media LLC, 2018.
-
Abstract
- The phenotype of Escherichia coli is governed by global transcriptional regulators under variable environmental conditions. Fnr, ArcA, IhfA-B, Crp, and Fis are amongst the major global transcription regulators that change their activity across the range of aeration, hence forming the core transcriptional network responsible for survival under changing aeration conditions. Effect of deletion of these global transcription factors on the kinetics of cell growth and mixed acid production under anaerobic fermentation conditions has not been characterized. To quantify the kinetic parameters in the absence of global transcription factors, experiments were performed using single deletion mutants of the above-mentioned global transcription regulators. The absence of global transcription regulators resulted in a relatively higher glucose uptake rate than that required for the observed growth rate. This further resulted in a higher yield of mixed acids per unit biomass in mutants as compared to the parent strain (E. coli BW25113). Thus, the increased channeling of carbon towards mixed acid secretion resulted in a lower growth rate in the mutants.
- Subjects :
- INTEGRATION HOST FACTOR
0301 basic medicine
FNR
030106 microbiology
Mutant
CULTURES
medicine.disease_cause
Biochemistry
Microbiology
03 medical and health sciences
Transcription (biology)
Escherichia coli
Genetics
medicine
Secretion
Anaerobiosis
Molecular Biology
Transcription factor
GENE-EXPRESSION
Global transcription factors
Cell growth
Chemistry
Escherichia coli Proteins
E. coli
ARCA
DNA
HOMODIMERS
Gene Expression Regulation, Bacterial
General Medicine
Kinetic analysis
HIMD SUBUNITS
Kinetics
030104 developmental biology
SYSTEMS BIOLOGY
Fermentation
GROWTH
Anaerobic fermentation
Aeration
Transcription Factors
Subjects
Details
- ISSN :
- 1432072X and 03028933
- Volume :
- 200
- Database :
- OpenAIRE
- Journal :
- Archives of Microbiology
- Accession number :
- edsair.doi.dedup.....791bde7506f5a688eb899304956aebb8