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Noninvasive three-state sleep-wake staging in mice using electric field sensors
- Source :
- J Neurosci Methods
- Publication Year :
- 2020
-
Abstract
- Study Objective Validate a novel method for sleep-wake staging in mice using noninvasive electric field (EF) sensors. Methods Mice were implanted with electroencephalogram (EEG) and electromyogram (EMG) electrodes and housed individually. Noninvasive EF sensors were attached to the exterior of each chamber to record respiration and other movement simultaneously with EEG, EMG, and video. A sleep-wake scoring method based on EF sensor data was developed with reference to EEG/EMG and then validated by three expert scorers. Additionally, novice scorers without sleep-wake scoring experience were self-trained to score sleep using only the EF sensor data, and results were compared to those from expert scorers. Lastly, ability to capture three-state sleep-wake staging with EF sensors attached to traditional mouse home-cages was tested. Results EF sensors quantified wake, rapid eye movement (REM) sleep, and non-REM sleep with high agreement (>93%) and comparable inter- and intra-scorer error as EEG/EMG. Novice scorers successfully learned sleep-wake scoring using only EF sensor data and scoring criteria, and achieved high agreement with expert scorers (>91%). When applied to traditional home-cages, EF sensors enabled classification of three-state (wake, NREM and REM) sleep-wake independent of EEG/EMG. Conclusions EF sensors score three-state sleep-wake architecture with high agreement to conventional EEG/EMG sleep-wake scoring 1) without invasive surgery, 2) from outside the home-cage, and 3) and without requiring specialized training or equipment. EF sensors provide an alternative method to assess rodent sleep for animal models and research laboratories in which EEG/EMG is not possible or where noninvasive approaches are preferred.
- Subjects :
- 0301 basic medicine
Computer science
Sleep wake
Scoring criteria
Sleep, REM
Electroencephalography
Non-rapid eye movement sleep
Article
03 medical and health sciences
Mice
0302 clinical medicine
Electric field sensor
medicine
Animals
Wakefulness
Alternative methods
medicine.diagnostic_test
business.industry
Electromyography
General Neuroscience
Eye movement
Pattern recognition
030104 developmental biology
Invasive surgery
Artificial intelligence
Sleep Stages
business
Sleep
030217 neurology & neurosurgery
psychological phenomena and processes
Subjects
Details
- ISSN :
- 1872678X
- Volume :
- 344
- Database :
- OpenAIRE
- Journal :
- Journal of neuroscience methods
- Accession number :
- edsair.doi.dedup.....79118a821cba73bf7148f534cc41242f