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Kushenol C Prevents Tert-Butyl Hydroperoxide and Acetaminophen-Induced Liver Injury
- Source :
- Molecules, Volume 26, Issue 6, Molecules, Vol 26, Iss 1635, p 1635 (2021)
- Publication Year :
- 2021
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2021.
-
Abstract
- Sophora flavescens, also known as Kushen, has traditionally been used as a herbal medicine. In the present study we evaluated the ameliorative effects of kushenol C (KC) from S. flavescens against tBHP (tert-Butyl hydroperoxide)-induced oxidative stress in hepatocellular carcinoma (HEPG2) cells and acetaminophen (APAP)-induced hepatotoxicity in mice. KC pretreatment protected the HEPG2 cells against oxidative stress by reducing cell death, apoptosis and reactive oxygen species (ROS) generation. KC pretreatment also upregulated pro-caspase 3 and GSH (glutathione) as well as expression of 8-Oxoguanine DNA Glycosylase (OGG1) in the HEPG2 cells. The mechanism of action was partly related by KC’s activation of Akt (Protein kinase B (PKB)) and Nrf2 (Nuclear factor (erythroid-derived 2)-like 2) in the HepG2 cells. In in vivo investigations, coadministration of mice with KC and APAP significantly attenuated APAP-induced hepatotoxicity and liver damage, as the serum enzymatic activity of aspartate aminotransferase and alanine aminotransferase, as well as liver lipid peroxidation and cleaved caspase 3 expression, were reduced in APAP-treated mice. Coadministration with KC also up-regulated antioxidant enzyme expression and prevented the production of proinflammatory mediators in APAP-treated mice. Taken together, these results showed that KC treatment has potential as a therapeutic agent against liver injury through the suppression of oxidative stress.
- Subjects :
- Male
Antioxidant
antioxidant
medicine.medical_treatment
Pharmaceutical Science
Pharmacology
medicine.disease_cause
Antioxidants
Analytical Chemistry
Mice
chemistry.chemical_compound
tert-Butylhydroperoxide
Drug Discovery
OGG1
Liver injury
chemistry.chemical_classification
Mice, Inbred BALB C
0303 health sciences
biology
030302 biochemistry & molecular biology
digestive, oral, and skin physiology
Alanine Transaminase
Hep G2 Cells
Glutathione
Liver
Chemistry (miscellaneous)
Chemical and Drug Induced Liver Injury, Chronic
Molecular Medicine
Sophora
Signal Transduction
liver injury
NF-E2-Related Factor 2
Herbal Medicine
Caspase 3
Article
kushenol C
Nrf2
lcsh:QD241-441
03 medical and health sciences
lcsh:Organic chemistry
Cell Line, Tumor
medicine
Animals
Humans
Aspartate Aminotransferases
Physical and Theoretical Chemistry
Acetaminophen
030304 developmental biology
Reactive oxygen species
Sophora flavescens
Plant Extracts
Akt
Organic Chemistry
medicine.disease
biology.organism_classification
Oxidative Stress
chemistry
Apoptosis
Lipid Peroxidation
Reactive Oxygen Species
Proto-Oncogene Proteins c-akt
Oxidative stress
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....790c28031b96865fa48978d440845b45
- Full Text :
- https://doi.org/10.3390/molecules26061635