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Duration of the initial TCR stimulus controls the magnitude but not functionality of the CD8+ T cell response
- Source :
- The Journal of Experimental Medicine
- Publication Year :
- 2006
- Publisher :
- Rockefeller University Press, 2006.
-
Abstract
- CD8+ T cells only require a brief stimulation with antigen in vitro to divide and differentiate into effector and memory cells upon transfer in vivo. The efficiency of clonal expansion and the functional characteristics of memory cells derived from briefly stimulated cells are poorly defined. We developed a system that allowed us to examine programming entirely in vivo. This was achieved by rapidly killing peptide-pulsed DCs carrying a diphtheria toxin receptor transgene with timed injections of diphtheria toxin without altering the course of an accompanying infection. The magnitude of clonal expansion, but not the functionality of the effector cells, correlated directly with the duration of antigen exposure. Furthermore, memory T cells were capable of mounting a secondary response, regardless of the length of antigen encounter during the primary response. These results indicate that the duration of initial antigen encounter influences the magnitude of the primary response, but does not program responsiveness during the secondary challenge.
- Subjects :
- Time Factors
Immunology
Receptors, Antigen, T-Cell
CD8-Positive T-Lymphocytes
Lymphocyte Activation
Article
Mice
03 medical and health sciences
0302 clinical medicine
Antigen
Animals
Immunology and Allergy
Cytotoxic T cell
Diphtheria Toxin
Transgenes
IL-2 receptor
Antigens
Antigen-presenting cell
Cell Proliferation
030304 developmental biology
Diphtheria toxin
0303 health sciences
CD40
biology
T-cell receptor
Dendritic Cells
Articles
Adoptive Transfer
Mice, Inbred C57BL
biology.protein
Immunologic Memory
CD8
030215 immunology
Subjects
Details
- ISSN :
- 15409538 and 00221007
- Volume :
- 203
- Database :
- OpenAIRE
- Journal :
- Journal of Experimental Medicine
- Accession number :
- edsair.doi.dedup.....79074b0e9c78ece2fc8c53516deb597d