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An Adenovirus-Based HIV Subtype B Prime/Boost Vaccine Regimen Elicits Antibodies Mediating Broad Antibody-Dependent Cellular Cytotoxicity Against Non-Subtype B HIV Strains
- Source :
- JAIDS Journal of Acquired Immune Deficiency Syndromes. 43:270-277
- Publication Year :
- 2006
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2006.
-
Abstract
- Although HIV subtype B predominates in North America and Western Europe, most HIV infections worldwide are non-subtype B. Globally effective AIDS vaccines need to elicit broad immunity against multiple HIV strains. In this study, 10 chimpanzees were intranasally primed sequentially with adenovirus type 5 (Ad5)-and Ad7-HIV MN env/rev recombinants and boosted twice intramuscularly with heterologous oligomeric HIV SF162 gpl40AV2 protein in MF59 adjuvant. Sera were evaluated for binding, neutralizing, and antibody-dependent cellular cytotoxicity (ADCC) against HIV clades A, B, C, and CRF01_AE. The vaccine regimen elicited high-titered HIV subtype A, B, C and CRF01_AE gp120-binding antibodies. Sera from 7 of 10 vaccinated chimpanzees cross-neutralized the heterologous South African subtype C primary HIV TV-1 isolate. Significant cross-clade neutralization against other subtype A, C and E isolates was not observed. Sera from all animals mediated ADCC of cells coated with gp120 from HIV subtypes A and B. Nine of 10 animals also exhibited ADCC activity against HIV subtype C and CRF01_AE gp120-coated targets. This subtype B Ad-HIV recombinant prime/envelope protein boost regimen is a promising approach for eliciting broad ADCC activity against diverse HIV clades. Incorporating additional non-subtype B envelope genes and protein boosts in a multivalent strategy may be required to elicit broader neutralizing antibodies against non-subtype B HIV strains.
- Subjects :
- Pan troglodytes
viruses
medicine.medical_treatment
Genetic Vectors
Drug Evaluation, Preclinical
Immunization, Secondary
Heterologous
HIV Infections
Cross Reactions
HIV Antibodies
HIV Envelope Protein gp120
Biology
Virus
Adenoviridae
Acquired immunodeficiency syndrome (AIDS)
Neutralization Tests
Immunity
medicine
Animals
Pharmacology (medical)
AIDS Vaccines
Antibody-dependent cell-mediated cytotoxicity
Vaccines, Synthetic
virus diseases
Viral Vaccines
medicine.disease
biology.organism_classification
Virology
Recombinant Proteins
Infectious Diseases
Immunology
Lentivirus
HIV-1
biology.protein
Antibody
Adjuvant
Subjects
Details
- ISSN :
- 15254135
- Volume :
- 43
- Database :
- OpenAIRE
- Journal :
- JAIDS Journal of Acquired Immune Deficiency Syndromes
- Accession number :
- edsair.doi.dedup.....78f77ed761d0752bfa6f5548c6279f7f