Life Inside a Thin Section: Tomography

Electron microscopic tomography is proving useful to examine the organization of protein molecules in large molecular machines at critical neural sites, such as synapses. Methods are still in a state of flux, and many steps exist between a neuron and a molecular model of one of its synapses. Here, we show our approach for doing tomography on synaptic structure, hoping thereby to shed light generally on the emerging field of EM tomography.