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Rapid Detection of Bacteria from Blood with Surface-Enhanced Raman Spectroscopy
- Source :
- Analytical chemistry. 88(16)
- Publication Year :
- 2016
-
Abstract
- Traditional methods for identifying pathogens in bacteremic patients are slow (24–48+ h). This can lead to physicians making treatment decisions based on an incomplete diagnosis and potentially increasing the patient’s mortality risk. To decrease time to diagnosis, we have developed a novel technology that can recover viable bacteria directly from whole blood and identify them in less than 7 h. Our technology combines a sample preparation process with surface-enhanced Raman spectroscopy (SERS). The sample preparation process enriches viable microorganisms from 10 mL of whole blood into a 200 μL aliquot. After a short incubation period, SERS is used to identify the microorganisms. We further demonstrated that SERS can be used as a broad detection method, as it identified a model set of 17 clinical blood culture isolates and microbial reference strains with 100% identification agreement. By applying the integrated technology of sample preparation and SERS to spiked whole blood samples, we were able to correctly identify both Staphylococcus aureus and Escherichia coli 97% of the time with 97% specificity and 88% sensitivity.
- Subjects :
- Staphylococcus aureus
Surface Properties
Microorganism
Analytical chemistry
02 engineering and technology
Spectrum Analysis, Raman
01 natural sciences
Rapid detection
Article
Analytical Chemistry
medicine
Escherichia coli
Humans
Model set
Sample preparation
Blood culture
Whole blood
Chromatography
biology
medicine.diagnostic_test
Chemistry
010401 analytical chemistry
Surface-enhanced Raman spectroscopy
021001 nanoscience & nanotechnology
biology.organism_classification
0104 chemical sciences
0210 nano-technology
Bacteria
Subjects
Details
- ISSN :
- 15206882
- Volume :
- 88
- Issue :
- 16
- Database :
- OpenAIRE
- Journal :
- Analytical chemistry
- Accession number :
- edsair.doi.dedup.....78e990ebe59138976b83e057409d355e