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Seamless Combination of Fluorescence-Activated Cell Sorting and Hanging-Drop Networks for Individual Handling and Culturing of Stem Cells and Microtissue Spheroids
- Source :
- Analytical Chemistry, Anal Chem
- Publication Year :
- 2016
-
Abstract
- Open microfluidic cell culturing devices offer new possibilities to simplify loading, culturing, and harvesting of individual cells or microtissues due to the fact that liquids and cells/microtissues are directly accessible. We present a complete workflow for microfluidic handling and culturing of individual cells and microtissue spheroids, which is based on the hanging-drop network concept: The open microfluidic devices are seamlessly combined with fluorescence-activated cell sorting (FACS), so that individual cells, including stem cells, can be directly sorted into specified culturing compartments in a fully automated way and at high accuracy. Moreover, already assembled microtissue spheroids can be loaded into the microfluidic structures by using a conventional pipet. Cell and microtissue culturing is then performed in hanging drops under controlled perfusion. On-chip drop size control measures were applied to stabilize the system. Cells and microtissue spheroids can be retrieved from the chip by using a parallelized transfer method. The presented methodology holds great promise for combinatorial screening of stem-cell and multicellular-spheroid cultures. [Figure: see text]
- Subjects :
- 0301 basic medicine
Chemistry
Drop (liquid)
Stem Cells
Microfluidics
Pipette
Spheroid
Cell Culture Techniques
Nanotechnology
Cell sorting
Microfluidic Analytical Techniques
Flow Cytometry
HCT116 Cells
Article
Analytical Chemistry
03 medical and health sciences
Fluorescence-Activated Cell Sorting
030104 developmental biology
Cell culture
Spheroids, Cellular
Humans
Stem cell
Particle Size
Subjects
Details
- Volume :
- 88
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Analytical Chemistry
- Accession number :
- edsair.doi.dedup.....78dc602ffcac3ff16551141d18baa2ad
- Full Text :
- https://doi.org/10.1021/acs.analchem.5b03513