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Adoptive transfer of type 1 regulatory T cells suppressed the development of airway hyperresponsiveness in ovalbumin-induced airway inflammation model mice
- Source :
- Journal of Pharmacological Sciences, Vol 141, Iss 4, Pp 139-145 (2019)
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Type 1 regulatory T (Tr1) cells are CD4+ T cells that produce a large amount of IL-10, an anti-inflammatory cytokine. However, it has not been fully elucidated whether Tr1 cells suppress allergic asthma. In this study, the effects of adoptive transfer of in vitro-induced Tr1 cells on allergic asthma were evaluated. Splenocytes from ovalbumin (OVA)-sensitized BALB/c mice were cultured with OVA, IL-21, IL-27, and TGF-β. After culture, IL-10-producing CD4+ T cells were isolated by Dynabeads mouse CD4 and IL-10 secretion assay, and analyzed by flow cytometry. Purified Tr1 cells (IL-10+ CD4+ T cells) were intravenously injected into OVA-sensitized BALB/c mice. The recipient mice were intratracheally challenged with OVA. Airway hyperresponsiveness to methacholine was assessed by the forced oscillation technique, followed by bronchoalveolar lavage (BAL). Almost all of the induced IL-10-producing CD4+ T cells were negative for interferon-γ, IL-4, IL-17A, and forkhead box P3, suggesting that the cells were Tr1 cells. The adoptive transfer of Tr1 cells significantly suppressed the development of airway hyperresponsiveness, and increases in IL-5, eosinophils, and neutrophils in BAL fluid. In conclusion, we demonstrated that Tr1 cells suppressed allergic asthma in mice. Keywords: Asthma, Airway hyperresponsiveness, IL-10, Immunotherapy, T cell
- Subjects :
- 0301 basic medicine
Adoptive cell transfer
Ovalbumin
medicine.medical_treatment
T cell
Anti-Inflammatory Agents
T-Lymphocytes, Regulatory
Flow cytometry
Dynabeads
Mice
03 medical and health sciences
0302 clinical medicine
Respiratory Hypersensitivity
medicine
Animals
Humans
Inflammation
Pharmacology
Mice, Inbred BALB C
biology
medicine.diagnostic_test
Chemistry
lcsh:RM1-950
Forkhead Transcription Factors
Immunotherapy
respiratory system
Adoptive Transfer
Interleukin 10
Treatment Outcome
lcsh:Therapeutics. Pharmacology
030104 developmental biology
Cytokine
medicine.anatomical_structure
Immunology
biology.protein
Molecular Medicine
Female
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 13478613
- Volume :
- 141
- Database :
- OpenAIRE
- Journal :
- Journal of Pharmacological Sciences
- Accession number :
- edsair.doi.dedup.....78da9025a72483df2c6263c014f33494